Identification and expression of the medaka inhibin βE subunit
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Activin E, a member of the TGF-β super family, is a protein dimer of mature inhibin βE subunits. Recently, it is reported that hepatic activin E may act as a hepatokine that alter whole body energy/glucose metabolism in human. However, orthologues of the activin E gene have yet to be identified in lower vertebrates, including fish. Here, we cloned the medaka (Oryzias latipes) activin E cDNA from liver. Among all the mammalian inhibin β subunits, the mature medaka activin E amino acid sequence shares the highest homology with mammalian activin E. Recombinant expression studies suggest that medaka activin E, the disulfide–bound mature form of mature inhibin βE subunits, may exert its effects in a way similar to that in mammals. Although activin E mRNA is predominantly expressed in liver in mammals, it is ubiquitously expressed in medaka tissues. Since expression in the liver was enhanced after a high fat diet, medaka activin E may be associated with energy/glucose metabolism, as shown in mice and human.
KeywordsActivin cDNA cloning Energy homeostasis Disease model Inhbe Recombinant protein
The authors thank Dr. R. Nakao for helpful discussions.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 2.Klaunig JE, Barut BA, Goldblatt PJ (1984) Preliminary studies on the usefulness of medaka, Oryzias latipes, embryos in carcinogenicity testing. Natl Cancer Inst Monogr 65:155–161Google Scholar
- 9.Wiater E, Vale W (2008) Activins and inhibins. In: Derynck R, Miyazono K (eds) The TGF-β Family. Cold Spring Harbor Laboratory Press, New York, pp 79–120Google Scholar
- 13.Hashimoto O, Funaba M, Sekiyama K, Doi S, Shindo D, Sato R, Itoi H, Oiwa H, Morita M, Suzuki C, Sugiyama M, Yamakawa N, Takada H, Matsumura S, Inoue K, Oyadomari S, Sugino H, Kurisaki A (2018) Activin E controls energy homeostasis in both brown and white adipose tissues as a hepatokine. Cell Rep 25:1193–1203CrossRefGoogle Scholar
- 14.Sugiyama M, Kikuchi A, Misu H, Igawa H, Ashihara M, Kushima Y, Honda K, Suzuki Y, Kawabe Y, Kaneko S, Takamura T (2018) Inhibin βE (INHBE) is a possible insulin resistance-associated hepatokine identified by comprehensive gene expression analysis in human liver biopsy samples. PLoS ONE 13:e0194798CrossRefGoogle Scholar
- 15.Duran EM, Shapshak P, Worley J, Minagar A, Ziegler F, Haliko S, Moleon-Borodowsky I, Haslett PA (2005) Presenilin-1 detection in brain neurons and FOXP3 in peripheral blood mononuclear cells: normalizer gene selection for real time reverse transcriptase pcr using the delta delta Ct method. Front Biosci 10:2955–2965CrossRefGoogle Scholar
- 26.Bailly-Maitre B, Belgardt BF, Jordan SD, Coornaert B, von Freyend MJ, Kleinridders A, Mauer J, Cuddy M, Kress CL, Willmes D, Essig M, Hampel B, Protzer U, Reed JC, Brü̈ning JC (2010) Hepatic Bax inhibitor-1 inhibits IRE1 and protects from obesity-associated insulin resistance and glucose intolerance. J Biol Chem 285:6198–6207CrossRefGoogle Scholar