Identification of porcine CTLA4 gene polymorphism and their association with piglet diarrhea and performance traits

  • Xiaowen Gao
  • Dongchun Guo
  • Mingxing Kou
  • Guiling Xing
  • Andong Zha
  • Xiuqin Yang
  • Xibiao Wang
  • Shengwei Di
  • Jiancheng Cai
  • Buyue Niu
Original Article


The objective of this study was to evaluate the association between the cytotoxic T-lymphocyte-associated antigen 4 (CTLA4) gene and piglet diarrhea. In this study, the mRNA expression of the CTLA4 gene increased significantly in IPEC-J2 cells after Escherichia coli K88 infection. Single nucleotide polymorphisms (SNPs) located in the 5′ flanking region (SNPs g.107281989C>T) and 3′-untranslated region (3′-UTR; SNPs g.107288753C>A) were identified, and they were in linkage disequilibrium in both Min pigs and the Landrace population. Association analysis showed that Landrace piglets with a TT or AA genotype had a lower diarrhea index, and AA animals had higher average daily gain when compared to CC pigs, respectively (p < 0.05). However, the relationship between SNPs and diarrhea and performance traits in the Min population was not significant. Haplotype analysis indicated that the TC haplotype had the lowest diarrhea index. The 5′ flanking deletion assay suggested that SNP g.107281989C>T was a molecular marker instead of the functional marker. This research demonstrated that genetic variances in the CTLA4 gene had significant effects on Landrace piglet diarrhea resistance.


Pig CTLA4 gene Polymorphism Haplotype Diarrhea 



We thank staff from Lanxi Breeding Farm (Lanxi, Heilongjiang, China) for managing the animals. This study was supported financially by National Natural Science Foundation of China (31301935), Science and Technology Foundation of Education Department, Heilongjiang Province (12541005).

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Ethical approval

This study meets the criteria of Institutional Animal Care and approved by the Committee of Northeast Agricultural University.

Informed consent

The owner of the animals was informed and consented in writing.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.College of Animal Science and TechnologyNortheast Agricultural UniversityHarbinChina
  2. 2.State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research InstituteChinese Academy of Agricultural Sciences (CAAS)HarbinChina
  3. 3.Lanxi breeding FarmLanxiChina

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