Novel flourescent spiroborate esters: potential therapeutic agents in in vitro cancer models
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The current treatment system in cancer therapy, which includes chemotherapy/radiotherapy is expensive and often deleterious to surrounding healthy tissue. Presently, several medicinal plants and their constituents are in use to manage the development and progression of these diseases.They have been found effective, safe, and less expensive. In the present study, we are proposing the utility of a new class of curcumin derivative, Rubrocurcumin, the spiroborate ester of curcumin with boric acid and oxalic acid (1:1:1), which have enhanced biostability for therapeutic applications. In vitro cytocompatibility of this drug complex was analysed using MTT assay, neutral red assay, lactate dehydrogenase assay in 3T3L1 adipocytes. Anti tumour activity of this drug complex on MCF7 and A431 human cancer cell line was studied by morphological analysis using phase contrast microscopy, Hoechst staining and cell cycle analysis by FACS. To explore the chemotherapeutic effect, the cytotoxic effect of this compound was also carried out. Rubrocurcumin is more biostable than natural curcumin in physiological medium. Our results prove that this curcumin derivative drug complex possess more efficacy and anti-cancer activity compared with curcumin. The findings out of this study suggests this novel compound as potential candidate for site targeted drug delivery.
KeywordsCurcumin Skin cancer Drug delivery Natural product Anti-cancer agent
The first author acknowledges Indian Council for Medical Research (ICMR) for the award of Senior Research fellowship to carry out this work.
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Conflict of interest
The authors confirm that there are no known conflicts of interest associated with this publication.
- 5.Sonavane K, Phillips J, Ekshyyan O, Moore-Medlin T, Gill JR, Rong X, Lakshmaiah RR, Abreo F, Boudreaux D, Clifford JL, Nathan CAO (2012) Topical curcuminbased cream is equivalent to dietary curcumin in a skin cancer model. J Skin Cancer. https://doi.org/10.1155/2012/147863 CrossRefPubMedPubMedCentralGoogle Scholar
- 12.Jeena J, Sudha Devi R, Balachandran S (2016) Kinetic analysis of thermal decomposition of rubrocurcumin. Acta Clienta Indica XLII C 2:121Google Scholar
- 19.Vijayalaksmi R, Sathyanarayana MN, Rao MVL (1981) Rubrocurcumin reaction and its use in microdetermination of certain organic acids. Indian J Chem 20B:907Google Scholar
- 21.Ji HF, Li XJ, Zhang HY (2009) Can thousands of years of ancient medical knowledge lead us to new and powerful drug combinations in the fight against cancer and dementia EMBO. Nat Prod Rep 10:194–200Google Scholar
- 23.Venu Gopal Y, Ravindranath A, Kalpana G, Rajkapoor B, Sreenivas S (2012) Antitumor and antioxidant activity of Diospyros peregrina against Dalton’s ascites lymphoma in rodents. Ann Biol Res 3:4985–4992Google Scholar