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Isolation and characterization of three skeletal troponin genes and association with growth-related traits in Exopalaemon carinicauda

  • Jiajia Wang
  • Qianqian Ge
  • Jitao Li
  • Zhao Chen
  • Jian Li
Original Article
  • 12 Downloads

Abstract

Growth is among the most important traits for animal breeding. Muscle growth is controlled by different cellular and molecular pathways and environments, and it also relies heavily on high-quality muscle contractions. The troponin complex, composed of troponin T (TnT), troponin C (TnC) and troponin I (TnI), plays a vital role in the regulation of muscle contraction. In this study, the cDNA of EcTnT, EcTnC and EcTnI of the ridgetail white prawn Exopalaemon carinicauda were cloned and characterized. The full length cDNA of EcTnT, EcTnC and EcTnI were 1 373 bp, 692 bp, and 1 475 bp, encoding a protein of 385, 150 and 193 amino acid residues, respectively. The expression of all genes was predominantly detected in abdominal muscle, while extremely lesser expressed in gill and hepatopancreas. Higher expression level of EcTnI was observed in heavier shrimp of the same age during different developmental stages, excepted for 120 days. Eleven single nucleotide polymorphisms (SNPs) were revealed in the three skeletal troponin genes, and only c.TnI66 A>G from EcTnI was significantly associated with both body weight and body length (P < 0.05). In summary, the result of this study suggested that EcTnI is growth-related gene of the troponin complex gene and the presence of SNP suggests that it could be a candidate gene for shrimp genetic improvement research.

Keywords

Exopalaemon carinicauda Troponin Gene expression Growth-related SNP 

Notes

Acknowledgements

This project was financially supported by the earmarked fund for National Key R & D Program of China (2018YFD0901302), Modern Agro-industry Technology Research System (No. CARS-48), The Program of Shandong Leading Talent (No. LNJY2015002), National Natural Science Foundation of China (No. 31472275) and Qingdao Industrial Development Program Science and Technology Benefit Special Project (17-3-3-62-nsh).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The collection and handling of the animals in this study was approved by the Animal Care and Use Committee at the Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, and all experimental animal protocols were carried out in accordance with national and institutional guidelines for the care and use of laboratory animals at the Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Key Laboratory for Sustainable Utilizatiyhuon of Marine Fisheries Resources, Yellow Sea Fisheries Research Institute, Ministry of Agriculture and RuralChinese Academy of Fishery SciencesQingdaoPeople’s Republic of China
  2. 2.Function Laboratory for Marine Fisheries Science and Food Production ProcessesQingdao National Laboratory for Marine Science and TechnologyQingdaoChina

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