Protective effects of anthocyanin against apoptosis and oxidative stress induced by arsanilic acid in DF-1 cells
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Anthocyanin is a natural plant pigment that acts as an antioxidant and scavenges free radicals. This study aimed to investigate the potential protective role of nightshade anthocyanin (NA), a natural flavonoid compound, against the arsanilic acid (ASA)-induced cell death of DF-1 cells. DF-1 cells were initially exposed to ASA, and then NA was applied to the treated cells. Cell viability, intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and apoptosis were examined. Results showed that NA inhibited the ASA-induced decrease in cell viability, increase in ROS, and loss of MMP in DF-1 cells. Moreover, caspase-3 activation was inhibited by ASA supplementation and NA attenuated the ASA-induced increase in the percentage of apoptotic cells. In summary, our study suggested that NA can enhance ASA-induced cytotoxicity and apoptosis, thereby providing a basis for the molecular mechanisms of NA-mediated protection.
KeywordsApoptosis Arsanilic acid Anthocyanin DF-1 cells
The project was supported by the National Key R&D Program (2016YFD0501208, 2016YFD0501007), National Nature Science Foundation of China (No. 31872535), Shandong Natural Science Foundation of China (ZR2018MC027, ZR2016CQ29) and Funds of Shandong “Double Tops” Program.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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