Protective effects of anthocyanin against apoptosis and oxidative stress induced by arsanilic acid in DF-1 cells

  • Ning Xie
  • Na Geng
  • Dong Zhou
  • Yuliang Xu
  • Kangping Liu
  • Yongxia LiuEmail author
  • Jianzhu LiuEmail author
Original Article


Anthocyanin is a natural plant pigment that acts as an antioxidant and scavenges free radicals. This study aimed to investigate the potential protective role of nightshade anthocyanin (NA), a natural flavonoid compound, against the arsanilic acid (ASA)-induced cell death of DF-1 cells. DF-1 cells were initially exposed to ASA, and then NA was applied to the treated cells. Cell viability, intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and apoptosis were examined. Results showed that NA inhibited the ASA-induced decrease in cell viability, increase in ROS, and loss of MMP in DF-1 cells. Moreover, caspase-3 activation was inhibited by ASA supplementation and NA attenuated the ASA-induced increase in the percentage of apoptotic cells. In summary, our study suggested that NA can enhance ASA-induced cytotoxicity and apoptosis, thereby providing a basis for the molecular mechanisms of NA-mediated protection.


Apoptosis Arsanilic acid Anthocyanin DF-1 cells 



The project was supported by the National Key R&D Program (2016YFD0501208, 2016YFD0501007), National Nature Science Foundation of China (No. 31872535), Shandong Natural Science Foundation of China (ZR2018MC027, ZR2016CQ29) and Funds of Shandong “Double Tops” Program.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.College of Veterinary MedicineShandong Agricultural UniversityTai’anChina
  2. 2.Research Center for Animal Disease Control EngineeringShandong Agricultural UniversityTai’anChina
  3. 3.Shandong Provincial Engineering Technology Research Center of Animal Disease Control and PreventionShandong Agricultural UniversityTai’anChina
  4. 4.College of Veterinary MedicineNorthwest Agriculture and Forestry UniversityYanglingChina

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