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Superparamagnetic iron oxide nanoparticles as a tool to track mouse neural stem cells in vivo

  • Ricardo Luiz Azevedo-Pereira
  • Bárbara Rangel
  • Fernanda Tovar-Moll
  • Emerson Leandro Gasparetto
  • Marcia Attias
  • Camila Zaverucha-do-Valle
  • Jasmin
  • Rosalia Mendez-Otero
Original Article
  • 77 Downloads

Abstract

Cell transplantation offers a promising approach in many neurological disorders. Neural stem (NS) cells are potential candidates for cell therapy. The ability to track the grafted cells in the host tissue will refine this therapy. Superparamagnetic iron oxide nanoparticles (SPION) have been suggested as a feasible method, but there is no consensus about its safety. Here we investigated the feasibility of label NS cells with SPION and track by MRI after transplantation into mouse striatum with SPION cells and its therapeutic effects by grafting the cells into mouse striatum. We demonstrated that SPION-labeled NS cells display normal patterns of cellular processes including proliferation, migration, differentiation and neurosphere formation. Transmission electron microscopy reveals SPION in the cytoplasm of the cells, which was confirmed by microanalysis. Neurons and astrocytes generated from SPION-labeled NS cells were able to carry nanoparticles after 7 days under differentiation. SPION-labeled NS cells transplanted into striatum of mice were detected by magnetic resonance imaging (MRI) and microscopy 51 days later. In agreement with others reports, we demonstrated that NS cells are able to incorporate SPION in vitro without altering the stemness, and can survive and be tracked by MRI after they have been grafted into mice striatum.

Keywords

Neural stem cells Superparamagnetic iron oxide nanoparticle Magnetic resonance imaging Cellular therapy Cell tracking 

Notes

Acknowledgements

This work was supported by CNPq, CAPES, FAPERJ, and the Ministry of Health (DECIT). We are grateful to Jennifer Vu (Stanford University) for helpful comments on the manuscript. The authors also thank Felipe Marins and Suelen Serio for technical assistance.

Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest to disclose.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Ricardo Luiz Azevedo-Pereira
    • 1
    • 4
  • Bárbara Rangel
    • 1
  • Fernanda Tovar-Moll
    • 2
  • Emerson Leandro Gasparetto
    • 2
  • Marcia Attias
    • 1
  • Camila Zaverucha-do-Valle
    • 1
    • 5
  • Jasmin
    • 3
  • Rosalia Mendez-Otero
    • 1
  1. 1.Instituto de Biofísica Carlos Chagas FilhoUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Universidade Federal do Rio de JaneiroRio de JaneiroBrazil
  3. 3.Núcleo Multidisciplinar de Pesquisa em Biologia-Duque de CaxiasUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  4. 4.Department of NeurosurgeryStanford UniversityPalo AltoUSA
  5. 5.Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo CruzRio de JaneiroBrazil

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