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Molecular Biology Reports

, Volume 46, Issue 1, pp 59–66 | Cite as

Moderation of physiological responses in rice plants with Azolla under 2,4-Dichlorophenoxy acetic acid stress

  • Arnab Kumar De
  • Arijit Ghosh
  • Kankana Biswas
  • M. K. AdakEmail author
Original Article
  • 67 Downloads

Abstract

The present work highlights some preliminary observations on metabolism in rice (Oryza sativa Linn.) when an aquatic fern Azolla pinnata R.Br. was co-cultured under 2,4-Dichlorophenoxy acetic acid toxicity. We have observed the effects of Azolla in both fresh and dried forms. This work signifies the possible physiological changes of a crop plant by using Azolla as a bioremediator. In brief the herbicide 2,4-D is considered as stressor to rice plants and by applying the fresh and dried Azolla we investigate the changes occurred. The activities of different nitrogen metabolizing enzymes and reactive oxygen species were observed. On the other hand chlorophyll and carotenoids synthesis were retrieved by addition of fresh and dried Azolla mass over 2,4-D toxicity. Thus, the efficiency of fresh and dried Azolla mass was evaluated under herbicidal toxicity in rice. We evaluate the bio remediating role of Azolla plants against 2,4-D stress and conclude this species would also be supporting in supplementation of major nutrients to rice plants.

Keywords

Azolla Herbicide Aquatic fern Rice Nitrogen metabolism Reactive oxygen species 

Abbreviations

2,4-D

2,4-Dichlorophenoxy acetic acid

MS media

Murashige Skoog’s media

H2O2

Hydrogen peroxide

O2

Superoxide

KI

Potassium iodide

HgCl2

Mercuric chloride

FW

Fresh weight

EDTA

Ethylenediamine tetra acetic acid

DTT

Dithiothreitol

PMSF

Phenyl-methyl-sulfonyl-fluoride

PVPP

Polyvinyl polypyrrolidone

MgCl2

Magnesium chloride

MnCl2

Manganese chloride

DW

Dry weight

TCA

Tri-chloro acetic acid

ROS

Reactive oxygen species

Notes

Acknowledgements

The present work is financially supported by DST-PURSE programme on University of Kalyani, DST, Govt. of INDIA, New Delhi.

Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Plant Physiology and Plant Molecular Biology Research Unit, Department of BotanyUniversity of KalyaniKalyaniIndia

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