Molecular Biology Reports

, Volume 45, Issue 6, pp 2545–2553 | Cite as

Association between the PPARa and PPARGCA gene variations and physical performance in non-trained male adolescents

  • Farzad ZehsazEmail author
  • Hamed Abbasi Soltani
  • Roya Hazrati
  • Negin Farhangi
  • Amir Monfaredan
  • Mehri Ghahramani
Original Article


The purpose of the research was to examine if some genetic variations are associated with some endurance, power and speed performances (multi-stage 20-m shuttle run, standing broad jump, 20 m sprint test and Abalakov jump) in a group of 586 non-trained male adolescents (mean ± SD age: 13.20 ± 0.25 years). Polymorphisms in PPARa and PPARGC1A implicated in physical performance traits were analyzed. DNA was extracted and the samples were genotyped for PPARa and PPARGC1A polymorphisms by a PCR based method followed by gel electrophoresis. The discrepancies in the study phenotypes among variations of the PPARa and PPARGC1A polymorphisms were analyzed by one-way analysis of covariance (ANCOVA), after age, weight and height adjustment. To examine whether the genotype and allele frequencies between adolescents with high and low performances were different, we divided them into two groups: ≥ 90th and < 90th of the percentile. The genotype and allele frequencies between adolescents with high and low performances were compared with the Chi square test. Our analysis demonstrated the effects of the PPARa and PPARGC1A polymorphisms only on \({\text{V}}{{\text{O}}_{{2{\text{max} }}}}\) (p = 0.010 and p = 0.010 respectively). Also, we observed significant differences in PPARa and PPARGC1A genotypes (p = 0.034 and p = 0.024) or allele frequencies (p = 0.031 and p = 0.001) between groups for the multi-stage 20-m shuttle run test. Findings of this research suggest that both the PPARa and PPARGC1A polymorphisms are associated with estimating endurance-related phenotype and endurance capacity in male non-athletes adolescents.


Polymorphism Genotype SNPs Adolescent Phenotype 



The study was designed by FZ and NF; data were collected and analyzed by HAS, RH and AM; data interpretation and manuscript preparation were undertaken by FZ, NF and MG. All authors approved the final version of the paper.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This protocol and the informed consent forms were reviewed and approved by the medical sciences ethics committee of Islamic Azad University-Tabriz Branch (No: REC.1396.74) with respect to scientific content and compliance with applicable research and human subjects regulations. The names of approving committee were Dr. Fatemeh Afshari, Dr. Babak Abri Agdam, Dr. Zohreh Gatreh Samani, Dr. Mohammad Taghi Zadiyeh and Seyyed Babak Khalifeh Zadeh. All procedures performed in this study involving human participants were in accordance with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Physical Education and Sport Sciences, Tabriz BranchIslamic Azad UniversityTabrizIran
  2. 2.Department of Hematology, Faculty of Medicine, Tabriz BranchIslamic Azad UniversityTabrizIran

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