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Molecular Biology Reports

, Volume 45, Issue 6, pp 2455–2467 | Cite as

Bioguided isolation, characterization and media optimization for production of Lysolipins by actinomycete as antimicrobial compound against Xanthomonas citri subsp. citri

  • Júlia Pereira Rodrigues
  • Ana Paula Ferranti Peti
  • Fernanda Salés Figueiró
  • Izadora de Souza Rocha
  • Vinicius Ricardo Acquaro Junior
  • Tamires Garcia Silva
  • Itamar Soares de Melo
  • Franklin Behlau
  • Luiz Alberto Beraldo Moraes
Original Article

Abstract

Citrus Canker disease is one of the most important disease in citrus production worldwide caused by gram-negative bacterial pathogen Xanthomonas citri subsp. citri, leading to great economic losses. Currently, a spray of copper-based bactericides is the primary measure for citrus canker management. However, these measures can lead to the contamination of soil by metal contamination, but also the development of copper-resistant Xanthomonas populations. Considering the need to discovery new alternatives to control the citrus canker disease, actinomycetes isolated from the Brazilian Caatinga biome and their crude extracts were tested against different strains of Xanthomonas citri subsp. citri. Streptomyces sp. Caat 1-54 crude extract showed the highest antibiotic activity against Xcc. The crude extract dereplication was performed by LC–MS/MS. Through bioassay-guided fractionation strategy, the antimicrobial activity was assigned to Lysolipins, showing a MIC around 0.4–0.8 µg/mL. Growth media optimization using statistical experimental design increased the Lysolipins production in three-fold production. The preventive and curative effects of the optimized crude extract obtained by experimental design of Caat-1-54 against citrus canker were evaluated in potted ‘Pera’ sweet orange nursery trees. Caat 1-54 extract was effective in preventing new infections by Xcc on leaves but was not able to reduce Xcc population in pre-established citrus canker lesions. Streptomyces sp. Caat 1-54 extract is a promising, environmentally-friendly source of antimicrobial compound to protect citrus trees against citrus canker.

Keywords

Actinobacteria Citrus canker control Secondary metabolites Antimicrobial agent Experimental design 

Abbreviations

CID

Collision-induced dissociation

RSM

Response surface methodology

MIC

Minimum inhibitory concentration

DMSO

Dimethyl sulfoxide

MTT

Thiazolyl blue tetrazolium bromide

TLC

Thin-layer chromatography

Notes

Funding

This work was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) - process (2016-22023-0) with financial support and fellowships.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

11033_2018_4411_MOESM1_ESM.docx (6 mb)
Supplementary material 1 (DOCX 6186 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Júlia Pereira Rodrigues
    • 1
  • Ana Paula Ferranti Peti
    • 1
  • Fernanda Salés Figueiró
    • 1
  • Izadora de Souza Rocha
    • 1
  • Vinicius Ricardo Acquaro Junior
    • 1
  • Tamires Garcia Silva
    • 2
  • Itamar Soares de Melo
    • 3
  • Franklin Behlau
    • 2
  • Luiz Alberto Beraldo Moraes
    • 1
  1. 1.Department of Chemistry, Faculty of Philosophy, Science and LettersUniversity of São PauloRibeirão PretoBrazil
  2. 2.FUNDECITRUS, Fundo de Defesa da CitriculturaAraraquaraBrazil
  3. 3.EMBRAPA, Empresa Brasileira de Pesquisa AgropecuáriaJaguariúnaBrazil

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