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Expression patterns and bioinformatic analysis of miR-1260a and miR-1274a in Prostate Cancer Tunisian patients

  • Rahma Said
  • Yoelsis Garcia-Mayea
  • Nesrine Trabelsi
  • Nouha Setti Boubaker
  • Cristina Mir
  • Ahlem Blel
  • Nidhal Ati
  • Rosanna Paciucci
  • Javier Hernández-Losa
  • Soumaya Rammeh
  • Amine Derouiche
  • Mohamed Chebil
  • Matilde E. LLeonart
  • Slah Ouerhani
Original Article
  • 42 Downloads

Abstract

Currently, microRNAs (miRs) represent great biomarkers in cancer due to their stability and their potential role in diagnosis, prognosis and therapy. This study aims to evaluate the expression levels of miRs-1260 and -1274a in prostate cancer (PC) samples and to identify their eventual targets by using bioinformatic analysis. In this project, we evaluated the expression status of miRs-1260 and -1274a in 86 PC patients and 19 controls by using real-time quantitative PCR and 2−ΔΔCt method. Moreover, we retrieved validated and predicted targets of miRs from several datasets by using the “multiMir” R/Bioconductor package. We have found that miRs-1260 and -1274a were over-expressed in PC patients compared to controls (p < 1 × 10−5). Moreover ROC curve for miRs-1260 and 1274a showed a good performance to distinguish between controls group and PC samples with an area under the ROC curve of 0.897 and 0.784 respectively. However, no significant association could be shown between these two miRs and clinical parameters such as PSA levels, Gleason score, tumor stage, D’Amico classification, lymph node metastasis statues, tumor recurrence, metastasis status and progression after a minimum of 5 years follow-up. Finally, a bioinformatic analysis revealed the association between these two miRs and several targets implicated in prostate cancer initiation pathways.

Keywords

MiR-1260 MiR-1274a Over-expression Epigenetic Prostate cancer Tunisia 

Notes

Acknowledgements

We would to thank to the medical team of Urology department and pathology anatomy and cytology department, Charles Nicolle Hospital, Tunis, Tunisia. We wish to thank to Rosa Somoza and Teresa Moline for their excellent technical assistance (Pathology Department at Vall d´Hebron Hospital), all members of Dr. LLeonart´s laboratory.

Funding

This work was supported by grants from the Instituto de Salud Carlos III, Grants PI12/01104 and PI15/01262 cofinanced by the European Regional Development Fund (ERDF) (ME LLeonart).

Compliance with ethical standards

Conflict of interest

All authors would like to declare that they have no conflict of interest.

Ethics approval

This work is approved by Ethic committee of Charles Nicolle-Tunis-Tunisia.

Informed consent

For this type of retrospective study formal consent is not required.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Laboratory of Protein Engineering and Bio-active MoleculesNational Institute of Applied Science and Technology - University of CarthageTunisTunisia
  2. 2.Biomedical Research in Cancer Stem Cells Group, Pathology DepartmentVall d’Hebron Research Institute (VHIR)BarcelonaSpain
  3. 3.Pathology Anatomy and Cytology DepartmentCharles Nicolle HospitalTunisTunisia
  4. 4.Urology DepartmentCharles Nicolle HospitalTunisTunisia
  5. 5.Faculty of Sciences of BizerteUniversity of CarthageTunisTunisia
  6. 6.Biomedical Research Group of Urology, Vall d’Hebron Research Institute (VHIR)Universitat Autònoma de BarcelonaBarcelonaSpain
  7. 7.Spanish Biomedical Research Network Centre in Oncology (CIBERONC)MadridSpain

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