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Molecular Biology Reports

, Volume 45, Issue 6, pp 1937–1945 | Cite as

Expression of the miR-190 family is increased under DDT exposure in vivo and in vitro

  • Tatiana S. KalininaEmail author
  • Vladislav V. Kononchuk
  • Vladimir Y. Ovchinnikov
  • Mikhail D. Chanyshev
  • Lyudmila F. Gulyaeva
Original Article
  • 133 Downloads

Abstract

A non-genotoxic insecticide dichlorodiphenyltrichloroethane (DDT), can affect mRNA and microRNA levels, however, its precise mechanism of action remains poorly understood. Using in silico methods we found that the rat miR-190 family is potentially regulated by CAR and ER receptors activated by DDT. We showed that exposure to DDT results in a dose- and organ-dependent increase in the expression of miR-190a, -190b in the liver, uterus, ovaries and mammary gland of female Wistar rats. Additionally, we demonstrate a decrease in protein product level of Tp53inp1, the target gene of these microRNAs, in the rat uterus. It is known that miR-190 is probably regulated by ER in humans, thus we measured the level of miR-190a, -190b in primary cultures of malignant and normal human endometrial cells treated with different doses of DDT. We detected an increase in miR-190b level in normal endometrial cells under DDT exposure. Thus, our results indicate that DDT exposure lead to change in the expression of oncogenic miR-190 family and its target gene Tp53inp1 which may be due to activation of CAR and ER.

Keywords

MicroRNA Dichlorodiphenyltrichloroethane Xenobiotics Constitutive androstane receptor Estrogen receptor 

Abbreviations

miR

MicroRNA

COP

Chlororganic pesticide

EDC

Endocrine disrupting chemical

DDT

Dichlorodiphenyltrichloroethane

ER

Estrogen receptor

CAR

Constitutive androstane receptor

PBREM

Phenobarbital-responsive enhancer module

ERE

Estrogen response element

nt

Nucleotide

Notes

Acknowledgements

This work is supported by the Russian Science Foundation, Project # 15-15-30012.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All experimental procedures were approved by the Bioethics Committee of the Institute of Molecular Biology and Biophysics and carried out in accordance with Directive 2010/63/EU.

Supplementary material

11033_2018_4343_MOESM1_ESM.pdf (217 kb)
Supplementary material 1 (PDF 216 KB)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Institute of Molecular Biology and BiophysicsNovosibirskRussia
  2. 2.Novosibirsk State UniversityNovosibirskRussia
  3. 3.Institute of Cytology and GeneticsNovosibirskRussia

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