Vicenin-2: a potential radiosensitizer of non-small cell lung cancer cells
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Non-small cell lung cancer (NSCLC) is a major form of cancer and is resistant to chemo- and radio-therapy. Vicenin-2 (VCN-2) is a flavonoid obtained from Ocimum sanctum L. and it has been reported to have radioprotective and anti-cancer properties. This study was conducted to check for the radiosensitizing potential of VCN-2 in the NSCLC cell line, NCI-H23. NCI-H23 cells were exposed to VCN-2 singularly, and to X-rays with and without prior VCN-2 treatment. Cytotoxicity assay, cell proliferation assay, caspase-3 activity assay, DNA fragmentation assay and Western blotting for Rad50, MMP-2 and p21 were performed to investigate the radiosensitizing properties of VCN-2. Fibroblast survival assay was performed using HEK293T cells to check for any adverse effects of VCN-2 on normal fibroblast cell line. VCN-2 singularly and in combination with radiation reduced the surviving cancer cells, increased caspase-3 activity, increased DNA fragmentation, increased the levels of Rad50 and lowered levels of MMP-2 and p21 proteins while being non-toxic and radioprotective to the fibroblast cells. VCN-2 showed a potent radiosensitizing property while also showing a chemotherapeutic property against NSCLC cell line NCI-H23.
KeywordsVicenin-2 Radiosensitization Caspase-3 Rad50 MMP-2 P21
This work was supported by funds provided by Department of Science and Technology-Science and Engineering Research Board, New Delhi, India to Dr. L. Kma (SERB/F/3862/2014-15) and grant from University Grants Commission, New Delhi, India under Departmental Research Support-II & Departmental Research Support-III program to the Department of Biochemistry, NEHU, Shillong, India.
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Conflict of interest
The authors declare that there is no conflict of interest.