Molecular Biology Reports

, Volume 45, Issue 5, pp 1533–1538 | Cite as

Development and characterization of 22 polymorphic microsatellites of the Andean frog Telmatobius chusmisensis (Anura, Telmatobius) and cross amplification in seven Chilean species of the genus

  • Alejandra Fabres
  • Pablo Fibla
  • Carolina Araya
  • Michel Sallaberry
  • Marco A. MéndezEmail author
Short Communication


The genus Telmatobius Wiegmann, 1834 is composed of a wide variety of species and is one of the most diverse native components of the high-altitude Andean environments. The species of the genus present in Chile are considered as endangered, critically endangered or data deficient. We isolated and evaluated 44 microsatellites in 80 individuals of 8 species of Telmatobius present in Chile, obtaining 22 polymorphic microsatellite loci for Telmatobius chusmisensis. The cross-amplification test was successful in all other species tested. For the first time, microsatellite markers are described for Telmatobius. The description of these primers will be useful for further genetic studies for T. chusmisensis and other species of the same genus; allowing further analyses of population structuring, dispersal patterns, recent demographic history and population effective size. This information is also significant to undertake conservation actions for the species of the genus Telmatobius, since most species have conservation issues.


Telmatobius Microsatellites Conservation genetics IUCN 



We thank F. Cruz, M. Valladares, P. Sáez and K. Otalora for their help in fieldwork and L. Eaton for his comments on the manuscript. P. Fibla thanks CONICYT PCHA/Doctorado Nacional/2014-21140355. This study was funded by Proyecto CONICYT-FONDECYT 1140540 (MM), and authorized by the Servicio Agrícola y Ganadero Resolución Exenta # 3037 and #5679. All the procedures carried out in this study were approved by the Bioethics Committee of the University of Chile (CICUA) resolution 131004FCS-UCH/s.


This study was funded by Proyecto CONICYT-FONDECYT (1140540).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involved human participants and/or animals

This study was authorized by the Servicio Agrícola y Ganadero Resolución Exenta # 3037 and #5679 (CL) and by the Bioethics Committee of the University of Chile (CICUA) resolution 131004FCS-UCH/s.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Laboratorio de Genética y Evolución, Departamento de Ciencias Ecológicas, Facultad de CienciasUniversidad de ChileSantiagoChile
  2. 2.Instituto de Investigación AgropecuariasSantiagoChile
  3. 3.Laboratorio de Zoología de Vertebrados, Departamento de Ciencias Ecológicas, Facultad de CienciasUniversidad de ChileSantiagoChile

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