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Molecular Biology Reports

, Volume 45, Issue 5, pp 1523–1526 | Cite as

Microsatellite loci obtained by next generation sequencing on the sablefish (Anoplopoma fimbria)

  • Adonaji Madeleine Orozco-Ruiz
  • Carolina Galván-Tirado
  • Svetlana Yu. Orlova
  • Alexei M. Orlov
  • Francisco Javier García-De León
Short Communication
  • 126 Downloads

Abstract

Eleven microsatellite loci were developed and characterized for the sablefish, Anoplopoma fimbria. The markers were identified from sequences obtained by next generation sequencing. Thirty samples from Aleutians Islands were genotyped. The amplifications were performed with three different annealing temperature and amplification products were visualized in ABI 3500 Genetic Analyzer. No evidence for scoring errors was detected by stuttering or due large allele dropout and neither of the loci presented a high null allele frequency (> 0.2). The number means of alleles per locus was of 12.21 and mean of observed and expected heterozygosity were of 0.60 and 0.75 respectively. The sablefish represents a resource of high commercial value on the coasts of Japan, Russia, Canada and west coast of the United States and these new primers could be useful to future diversity and structure population studies.

Keywords

Microsatellites Sablefish Next generation sequencing Genetic diversity 

Notes

Acknowledgements

This work was carried out thanks to the financing granted by SAGARPA-INAPESCA through the Program of Innovation, Research, Technological Development and Education of Aquatic Genetic Resources 2015. We also thank CONACYT for the fellowship granted through the National Researcher System (SNI) program to AMOR.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

No required for our submission.

Informed consent

No required for our submission.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Laboratorio de Genética para la ConservaciónCentro de Investigaciones Biológicas del Noroeste (CIBNOR)La PazMexico
  2. 2.Russian Federal Research Institute of Fisheries and Oceanography (VNIRO)MoscowRussia
  3. 3.Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences (IPEE)MoscowRussia
  4. 4.Dagestan State University (DSU)MakhachkalaRussia
  5. 5.Caspian Institute of Biological ResourcesDagestan Scientific Center of the Russian Academy of Sciences (CIBR)MakhachkalaRussia
  6. 6.Tosk State University (TSU)TomskRussia

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