Advertisement

Molecular Biology Reports

, Volume 45, Issue 5, pp 689–697 | Cite as

Black tea affects obesity by reducing nutrient intake and activating AMP-activated protein kinase in mice

  • Shunshun Pan
  • Xuming Deng
  • Shili SunEmail author
  • Xingfei Lai
  • Lingli Sun
  • Qiuhua Li
  • Limin Xiang
  • Lingzhi ZhangEmail author
  • Yahui HuangEmail author
Original article

Abstract

The effects of certain tea components on the prevention of obesity in humans have been reported recently. However, whether Yinghong NO. 9 black tea consumption has beneficial effects on obesity are not known. Here, we obtained a Yinghong NO. 9 black tea infusion (Y9 BTI) and examined the anti-obesity effects of its oral administration. ICR mice were fed a standard diet supplemented with Y9 BTI at 0.5, 1.0, or 2.0 g/kg body weight for two weeks, and the body weight were recorded. HE staining was used to evaluate the effect of Y9 BTI on mice liver. Western blot analysis was used to detect the expression levels of related proteins in the mice liver and adipose. We found that the body weights of the mice in the control group were significantly higher than those of the mice in the middle and high dose groups. The results of western blot showed that Y9 BTI up-regulated the expression of liver kinase B1 (LKB1) and adenosine monophosphate-activated protein kinase (AMPK) and also increased in AMPK phosphorylation (p-AMPK) and LKB1 phosphorylation (p-LKB1). Y9 BTI significantly down-regulated Fas Cell Surface Death Receptor(FAS) and activated the phosphorylation of acetyl-CoA carboxylase (ACC). Furthermore, Y9 BTI (2.0 g/kg BW) down-regulated the expression of three factors (IL-1β, Cox-2, and iNOS). Altogether, Y9 BTI supplementation reduced the feed intake of mice and may prevent obesity by inhibiting lipid absorption. These results suggest that Y9 BTI may regulate adipogenic processes through the LKB1/AMPK pathway.

Keywords

Yinghong NO. 9 black tea Anti-obesity AMP-activated protein kinase Inflammation 

Abbreviations

Y9 BTI

Yinghong NO. 9 black tea infusion

ICR

Institute of Cancer Research

LKB1

Liver kinase B1

AMPK

Adenosine monophosphate-activated protein kinase

Notes

Acknowledgements

This work was financially supported by the Fund for Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation and Utilization (No. 2008A060301004), the Construction of industrial technology innovation platform for black tea of Guangdong big leaves species, the earmarked fund for Modern Agro-industry Technology Research System (No. 2016LM2151), the Science and Technology Planning Project of Guangdong Province (Nos. 2016B090918118 and 2017A070702004), and the President Foundation of Guangdong Academy of Agricultural Sciences (Grant Nos. 201534 and 201720).

Author contributions

The authors’ responsibilities were as follows—SP, LZ and YH conceived and designed the experiments. SP, XD and SS performed the experiments. XD and XL analyzed the data. XL and LS contributed reagents and participated in animal anatomy. QL wrote the paper, and LX, LZ and YH critically revised the manuscript. All authors approved the final version of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

IACUC: Tea Research Institute, Guangdong Academy of Agricultural Sciences Institutional Review Board Approval of Experimental Animals, an approval number is Tea (2015) No. 004. In strict accordance with the promulgated in Guangdong Province, “Guangdong Province Experimental Animal Management Regulations,” the implementation of the use of CO2 inhalation after the injection of carotid artery blood to death, and then collected the relevant tissue samples.

Supplementary material

11033_2018_4205_MOESM1_ESM.docx (16 kb)
Table S1 (DOCX 16 KB)

References

  1. 1.
    Pichard C, Plu-Bureau G, Neves ECM, Gompel A (2008) Insulin resistance, obesity and breast cancer risk. Maturitas 60:19–30.  https://doi.org/10.1016/j.maturitas.2008.03.002 CrossRefPubMedGoogle Scholar
  2. 2.
    Alshatwi AA, Al Obaaid MA, Al Sedairy SA, Ramesh E, Lei KY (2011) Black and green tea improves lipid profile and lipid peroxidation parameters in Wistar rats fed a high-cholesterol diet. J Physiol Biochem 67:95–104.  https://doi.org/10.1007/s13105-010-0053-3 CrossRefPubMedGoogle Scholar
  3. 3.
    Wojtowicz J, Lempicka A, Luczynski W, Szczepanski W, Zomerfeld A, Semeran K, Bossowski A (2017) Central aortic pressure, arterial stiffness and echocardiographic parameters of children with overweight/obesity and arterial hypertension. Adv Clin Exp Med 26:1399–1404.  https://doi.org/10.17219/acem/65485 CrossRefPubMedGoogle Scholar
  4. 4.
    Aoki M, Hagiwara S, Oshima K, Suzuki M, Sakurai A, Tahara Y, Nagao K, Yonemoto N, Yaguchi A, Morimura N (2018) Obesity was associated with worse neurological outcome among Japanese patients with out-of-hospital cardiac arrest. Intensive Care Med.  https://doi.org/10.1007/s00134-017-5042-3 CrossRefPubMedGoogle Scholar
  5. 5.
    Salas IH, Weerasekera A, Ahmed T, Callaerts-Vegh Z, Himmelreich U, D’Hooge R, Balschun D, Saido TC, De Strooper B, Dotti CG (2018) High fat diet treatment impairs hippocampal long-term potentiation without alterations of the core neuropathological features of Alzheimer disease. Neurobiol Dis.  https://doi.org/10.1016/j.nbd.2018.02.001 CrossRefPubMedGoogle Scholar
  6. 6.
    Sargolzaei J, Chamani E, Kazemi T, Fallah S, Soori H (2018) The role of adiponectin and adipolin as anti-inflammatory adipokines in the formation of macrophage foam cells and their association with cardiovascular diseases. Clin Biochem.  https://doi.org/10.1016/j.clinbiochem.2018.02.008 CrossRefPubMedGoogle Scholar
  7. 7.
    Garg SK, Maurer H, Reed K, Selagamsetty R (2014) Diabetes and cancer: two diseases with obesity as a common risk factor. Diabetes Obes Metab 16:97–110.  https://doi.org/10.1111/dom.12124 CrossRefPubMedGoogle Scholar
  8. 8.
    Zhang WL, Zhu L, Jiang JG (2014) Active ingredients from natural botanicals in the treatment of obesity. Obes Rev 15:957–967.  https://doi.org/10.1111/obr.12228 CrossRefPubMedGoogle Scholar
  9. 9.
    Ma W, Dai Y, Row KH (2018) Molecular imprinted polymers based on magnetic chitosan with different deep eutectic solvent monomers for the selective separation of catechins in black tea. Electrophoresis.  https://doi.org/10.1002/elps.201800034 CrossRefPubMedGoogle Scholar
  10. 10.
    Carraturo F, De Castro O (2018) Comparative assessment of the quality of commercial black and green tea using microbiology analyses. BMC Microbiol.  https://doi.org/10.1186/s12866-017-1142-z CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Mao X, Gu C, Chen D, Yu B, He J (2017) Oxidative stress-induced diseases and tea polyphenols. Oncotarget 8:81649–81661.  https://doi.org/10.18632/oncotarget.20887 CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Henning SM, Yang J, Hsu M, Lee RP, Grojean EM, Ly A, Tseng CH, Heber D, Li Z (2017) Decaffeinated green and black tea polyphenols decrease weight gain and alter microbiome populations and function in diet-induced obese mice. Eur J Nutr.  https://doi.org/10.1007/s00394-017-1542-8 CrossRefPubMedGoogle Scholar
  13. 13.
    Arya V, Taneja L (2015) Inhibition of salivary amylase by black tea in high-caries and low-caries index children: A comparative in vivo study. Ayu 36:278–282.  https://doi.org/10.4103/0974-8520.182743 CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Anandhan A, Tamilselvam K, Radhiga T, Rao S, Essa MM, Manivasagam T (2012) Theaflavin, a black tea polyphenol, protects nigral dopaminergic neurons against chronic MPTP/probenecid induced Parkinson’s disease. Brain Res 1433:104–113.  https://doi.org/10.1016/j.brainres.2011.11.021 CrossRefPubMedGoogle Scholar
  15. 15.
    You T, Ryan AS, Nicklas BJ (2004) The metabolic syndrome in obese postmenopausal women: relationship to body composition, visceral fat, and inflammation. J Clin Endocrinol Metab 89:5517–5522.  https://doi.org/10.1210/jc.2004-0480 CrossRefPubMedGoogle Scholar
  16. 16.
    Yi T, Zhu L, Peng WL, He XC, Chen HL, Li J, Yu T, Liang ZT, Zhao ZZ, Chen HB (2015) Comparison of ten major constituents in seven types of processed tea using HPLC-DAD-MS followed by principal component and hierarchical cluster analysis. LWT-Food Sci Technol 62:194–201CrossRefGoogle Scholar
  17. 17.
    Ng KW, Cao ZJ, Chen HB, Zhao ZZ, Zhu L, Yi T (2017) Oolong tea: a critical review of processing methods, chemical composition, health effects, and risk. Crit Rev Food Sci Nutr.  https://doi.org/10.1080/10408398.2017.1347556 CrossRefPubMedGoogle Scholar
  18. 18.
    Arner P (2000) Obesity: a genetic disease of adipose tissue? Br J Nutr 83(Suppl 1):S9-16PubMedGoogle Scholar
  19. 19.
    Andersson U, Filipsson K, Abbott CR, Woods A, Smith K, Bloom SR, Carling D, Small CJ (2004) AMP-activated protein kinase plays a role in the control of food intake. J Biol Chem 279:12005–12008.  https://doi.org/10.1074/jbc.C300557200 CrossRefPubMedGoogle Scholar
  20. 20.
    Lin CL, Huang HC, Lin JK (2007) Theaflavins attenuate hepatic lipid accumulation through activating AMPK in human HepG2 cells. J Lipid Res 48:2334–2343.  https://doi.org/10.1194/jlr.M700128-JLR200 CrossRefPubMedGoogle Scholar
  21. 21.
    Chan PT, Fong WP, Cheung YL, Huang Y, Ho WK, Chen ZY (1999) Jasmine green tea epicatechins are hypolipidemic in hamsters (Mesocricetus auratus) fed a high fat diet. J Nutr 129:1094–1101.  https://doi.org/10.1093/jn/129.6.1094 CrossRefPubMedGoogle Scholar
  22. 22.
    Kim MS, Park JY, Namkoong C, Jang PG, Ryu JW, Song HS, Yun JY, Namgoong IS, Ha J, Park IS, Lee IK, Viollet B, Youn JH, Lee HK, Lee KU (2004) Anti-obesity effects of alpha-lipoic acid mediated by suppression of hypothalamic AMP-activated protein kinase. Nat Med 10:727–733.  https://doi.org/10.1038/nm1061 CrossRefPubMedGoogle Scholar
  23. 23.
    Nishiumi S, Bessyo H, Kubo M, Aoki Y, Tanaka A, Yoshida K, Ashida H (2010) Green and black tea suppress hyperglycemia and insulin resistance by retaining the expression of glucose transporter 4 in muscle of high-fat diet-fed C57BL/6J mice. J Agric Food Chem 58:12916–12923.  https://doi.org/10.1021/jf102840w CrossRefPubMedGoogle Scholar
  24. 24.
    Fei W, Tian DR, Tso P, Han JS (2012) Diet-induced obese rats exhibit impaired LKB1-AMPK signaling in hypothalamus and adipose tissue. Peptides 35:23–30.  https://doi.org/10.1016/j.peptides.2012.02.003 CrossRefGoogle Scholar
  25. 25.
    Fediuc S, Gaidhu MP, Ceddia RB (2006) Regulation of AMP-activated protein kinase and acetyl-CoA carboxylase phosphorylation by palmitate in skeletal muscle cells. J Lipid Res 47:412–420.  https://doi.org/10.1194/jlr.M500438-JLR200 CrossRefPubMedGoogle Scholar
  26. 26.
    Wang X, Tian W (2001) Green tea epigallocatechin gallate: a natural inhibitor of fatty-acid synthase. Biochem Biophys Res Commun 288:1200–1206.  https://doi.org/10.1006/bbrc.2001.5923 CrossRefPubMedGoogle Scholar
  27. 27.
    Wang S, Noh SK, Koo SI (2006) Epigallocatechin gallate and caffeine differentially inhibit the intestinal absorption of cholesterol and fat in ovariectomized rats. J Nutr 136:2791–2796CrossRefGoogle Scholar
  28. 28.
    Hill AM, Coates AM, Buckley JD, Ross R, Thielecke F, Howe PR (2007) Can EGCG reduce abdominal fat in obese subjects? J Am Coll Nutr 26:396s–402sCrossRefGoogle Scholar
  29. 29.
    Klaus S, Pultz S, Thone-Reineke C, Wolfram S (2005) Epigallocatechin gallate attenuates diet-induced obesity in mice by decreasing energy absorption and increasing fat oxidation. Int J Obes 29:615–623.  https://doi.org/10.1038/sj.ijo.0802926 CrossRefGoogle Scholar
  30. 30.
    Wolfram S, Raederstorff D, Wang Y, Teixeira SR, Elste V, Weber P (2005) TEAVIGO (epigallocatechin gallate) supplementation prevents obesity in rodents by reducing adipose tissue mass. Ann Nutr Metab 49:54–63.  https://doi.org/10.1159/000084178 CrossRefPubMedGoogle Scholar
  31. 31.
    Civera M, Urios A, Garcia-Torres ML, Ortega J, Martinez-Valls J, Cassinello N, del Olmo JA, Ferrandez A, Rodrigo JM, Montoliu C (2010) Relationship between insulin resistance, inflammation and liver cell apoptosis in patients with severe obesity. Diabetes Metab Res Rev 26:187–192.  https://doi.org/10.1002/dmrr.1070 CrossRefPubMedGoogle Scholar
  32. 32.
    Auvichayapat P, Prapochanung M, Tunkamnerdthai O, Sripanidkulchai BO, Auvichayapat N, Thinkhamrop B, Kunhasura S, Wongpratoom S, Sinawat S, Hongprapas P (2008) Effectiveness of green tea on weight reduction in obese Thais: a randomized, controlled trial. Physiol Behav 93:486–491.  https://doi.org/10.1016/j.physbeh.2007.10.009 CrossRefPubMedGoogle Scholar

Bibliography

  1. 33.
    Yuan E, Duan X, Xiang L, Ren J, Lai X, Li Q, Sun L, Sun S (2018) Aged oolong tea reduces high-fat diet-induced fat accumulation and dyslipidemia by regulating the AMPK/ACC signaling pathway. Nutrients 10:187.  https://doi.org/10.3390/nu10020187 CrossRefPubMedCentralGoogle Scholar
  2. 34.
    Zhao W, Li Y, Ma W, Ge Y, Huang Y (2015) Study on quality components and sleep promoting effect of GABA black tea. Food Funct 6(10):3393–3398.  https://doi.org/10.1039/c5fo00265f CrossRefPubMedGoogle Scholar
  3. 35.
    Teng J, Zhou W, Zeng Z, Zhao W, Huang Y, Zhang X (2017) Quality components and antidepressant-like effects of GABA green tea. Food Funct 8(9):3311–3318.  https://doi.org/10.1039/c7fo01045a CrossRefPubMedGoogle Scholar
  4. 36.
    Mei X, Chen Y, Zhang L, Fu X, Wei Q, Grierson D, Zhou Y, Huang Y, Dong F, Yang Z (2016) Dual mechanisms regulating glutamate decarboxylases and accumulation of gamma-aminobutyric acid in tea (Camellia sinensis) leaves exposed to multiple stresses. Sci Rep 6:23685.  https://doi.org/10.1038/srep23685 CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Tea Science, College of HorticultureSouth China Agricultural UniversityGuangzhouPeople’s Republic of China
  2. 2.Tea Research InstituteGuangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation & UtilizationGuangzhouPeople’s Republic of China

Personalised recommendations