Anticancer effect of fermented Insampaedok-san in human colon cancer cells: a pilot study

Short Communication
  • 32 Downloads

Abstract

Insampaedok-san (IPS) is one of the East Asian traditional medicines which have been prescribed for hundreds of years to treat common cold and headache. Although many herbs and prescriptions are known to have significant activities against diseases, only a limited number of reports and scientific evidences on their efficacies are available. To identify anticancer effect against colon cancer, traditional prescription IPS and its fermented IPS (FIPS) were examined by in vitro molecular biological analysis. IPS water extract was fermented, lyophilized, and examined by cytotoxicity, cell cycle, and western blot assays, using cancer cell lines. Resultantly, FIPS showed significant cytotoxicities inducing caspase dependent apoptosis and activation of caspase-3 (CASP3) and poly (ADP-ribose) polymerase (PARP) cleavage in colon cancer cell line SW620. These findings can be useful for development of anticancer functional food or complementary and alternative medicine, with remaining in-depth molecular functional studies.

Keywords

Colon cancer Fermentation Insampaedok-san Traditional medicine 

Notes

Acknowledgements

This work was supported by Grant (No. K13050) from the Korea Institute of Oriental Medicine funded by the Ministry of Science and ICT (Information and Communication Technologies), Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interests.

Supplementary material

11033_2018_4171_MOESM1_ESM.tif (499 kb)
Supplementary material 1 (TIF 499 KB)

References

  1. 1.
    Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A (2015) Global cancer statistics, 2012. CA Cancer J Clin 65(2):87–108CrossRefPubMedGoogle Scholar
  2. 2.
    Ribeiro-Varandas E, Ressurreição F, Viegas W, Delgado M (2014) Cytotoxicity of Eupatorium cannabinum L. ethanolic extract against colon cancer cells and interactions with bisphenol A and doxorubicin. BMC Complement Altern Med 14:264CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Yim NH, Jung YP, Kim A, Ma CJ, Cho WK, Ma JY (2013) Oyaksungisan, a traditional herbal formula, inhibits cell proliferation by induction of autophagy via JNK activation in human colon cancer cells. Evid Based Complement Altern Med 2013:231874Google Scholar
  4. 4.
    Zhang L, Cai Q, Lin J, Fang Y, Zhan Y, Shen A et al (2014) Chloroform fraction of Scutellaria barbata D. Don promotes apoptosis and suppresses proliferation in human colon cancer cells. Mol Med Rep 9(2):701–706CrossRefPubMedGoogle Scholar
  5. 5.
    Zorofchian Moghadamtousi S, Karimian H, Rouhollahi E, Paydar M, Fadaeinasab M, Abdul Kadir H (2014) Annona muricata leaves induce G1 cell cycle arrest and apoptosis through mitochondria-mediated pathway in human HCT-116 and HT-29 colon cancer cells. J Ethnopharmacol 156:277–289CrossRefPubMedGoogle Scholar
  6. 6.
    Jayaprakasha GK, Mandadi KK, Poulose SM, Jadegoud Y, Nagana Gowda GA, Patil BS (2008) Novel triterpenoid from Citrus aurantium L. possesses chemopreventive properties against human colon cancer cells. Bioorg Med Chem 16(11):5939–5951CrossRefPubMedGoogle Scholar
  7. 7.
    de la Cruz J, Kim DH, Hwang SG (2014) Anticancer effects of Cnidium officinale Makino extract mediated through apoptosis and cell cycle arrest in the HT-29 human colorectal cancer cell line. Asian Pac J Cancer Prev 15(2):5117–5121PubMedGoogle Scholar
  8. 8.
    Lee CK, Park KK, Lim SS, Park JH, Chung WY (2007) Effects of the licorice extract against tumor growth and cisplatin-induced toxicity in a mouse xenograft model of colon cancer. Biol Pharm Bull 30(11):2191–2195CrossRefPubMedGoogle Scholar
  9. 9.
    Lee MA, Kim WK, Park HJ, Kang SS, Lee SK (2013) Anti-proliferative activity of hydnocarpin, a natural lignan, is associated with the suppression of Wnt/β-catenin signaling pathway in colon cancer cells. Bioorg Med Chem Lett 23(20):5511–5514CrossRefPubMedGoogle Scholar
  10. 10.
    Nakamura Y, Hasegawa Y, Shirota K, Suetome N, Nakamura T, Chomnawang MT et al (2014) Differentiation-inducing effect of piperitenone oxide, a fragrant ingredient of spearmint (Mentha spicata), but not carvone and menthol, against human colon cancer cells. J Funct Foods 8:62–67CrossRefGoogle Scholar
  11. 11.
    Kang KA, Piao MJ, Kim KC, Zheng J, Yao CW, Cha JW et al (2013) Compound K, a metabolite of ginseng saponin, inhibits colorectal cancer cell growth and induces apoptosis through inhibition of histone deacetylase activity. Int J Oncol 43(6):1907–1914CrossRefPubMedGoogle Scholar
  12. 12.
    Kim JY, Park KW, Moon KD, Lee MK, Choi J, Yee ST et al (2008) Induction of apoptosis in HT-29 colon cancer cells by crude saponin from Platycodi Radix. Food Chem Toxicol 46(12):3753–3758CrossRefPubMedGoogle Scholar
  13. 13.
    Ríos JL (2011) Chemical constituents and pharmacological properties of Poria cocos. Planta Med 77(7):681–691CrossRefPubMedGoogle Scholar
  14. 14.
    Lin CB, Lin CC, Tsay GJ (2012) 6-Gingerol inhibits growth of colon cancer cell LoVo via induction of G2/M arrest. Evid Based Complement Altern Med 2012:326096Google Scholar
  15. 15.
    Ahn KS, Sim WS, Kim IH (1996) Decursin: a cytotoxic agent and protein kinase C activator from the root of Angelica gigas. Planta Med 62(1):7–9CrossRefPubMedGoogle Scholar
  16. 16.
    Kim HY, Kim JK, Choi JH, Jung JY, Oh WY, Kim DC et al (2010) Hepatoprotective effect of pinoresinol on carbon tetrachloride-induced hepatic damage in mice. J Pharmacol Sci 112(1):105–112CrossRefPubMedGoogle Scholar
  17. 17.
    Jung HW, Mahesh R, Park JH, Boo YC, Park KM, Park YK (2010) Bisabolangelone isolated from Ostericum koreanum inhibits the production of inflammatory mediators by down-regulation of NF-κB and ERK MAP kinase activity in LPS-stimulated RAW264.7 cells. Int Immunopharmacol 10(2):155–162CrossRefPubMedGoogle Scholar
  18. 18.
    Khan S, Shin EM, Choi RJ, Jung YH, Kim J, Tosun A et al (2011) Suppression of LPS-induced inflammatory and NF-κB responses by anomalin in RAW 264.7 macrophages. J Cell Biochem 112(8):2179–2188CrossRefPubMedGoogle Scholar
  19. 19.
    Wang BS, Huang GJ, Tai HM, Huang MH (2012) Antioxidant and anti-inflammatory activities of aqueous extracts of Schizonepeta tenuifolia Briq. Food Chem Toxicol 50(3–4):526–531CrossRefPubMedGoogle Scholar
  20. 20.
    Oh HL, Lee DK, Lim H, Lee CH (2010) HY253, a novel decahydrofluorene analog, from Aralia continentalis, induces cell cycle arrest at the G1 phase and cytochrome c-mediated apoptosis in human lung cancer A549 cells. J Ethnopharmacol 129(1):135–139CrossRefPubMedGoogle Scholar
  21. 21.
    Liu RY, Li JP (2014) Saikosaponin-d inhibits proliferation of human undifferentiated thyroid carcinoma cells through induction of apoptosis and cell cycle arrest. Eur Rev Med Pharmacol Sci 18(17):2435–2443PubMedGoogle Scholar
  22. 22.
    Xu C, Ji GE (2013) Bioconversion of flavones during fermentation in milk containing Scutellaria baicalensis extract by Lactobacillus brevis. J Microbiol Biotechnol 23(10):1422–1427CrossRefPubMedGoogle Scholar
  23. 23.
    Chang J (1999) Scientific evaluation of traditional Chinese medicine under DSHEA: a conundrum. J Altern Complement Med 5(2):181–189CrossRefPubMedGoogle Scholar
  24. 24.
    Fabricant DS, Farnsworth NR (2001) The value of plants used in traditional medicine for drug discovery. Environ Health Perspect 109(Suppl 1):69–75CrossRefPubMedPubMedCentralGoogle Scholar
  25. 25.
    Weon JB, Ma JY, Yang HJ, Ma CJ (2011) Quantitative analysis of compounds in fermented Insampaedok-san and their neuroprotective activity in HT22 cells. Nat Prod Sci 17(1):58–63Google Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Korean Medicine Application CenterKorea Institute of Oriental MedicineDaeguRepublic of Korea
  2. 2.Division of Applied Science, PMBBRCGyeongsang National UniversityJinjuRepublic of Korea

Personalised recommendations