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Molecular Biology Reports

, Volume 45, Issue 2, pp 99–108 | Cite as

Genetic structure and polymorphism analysis of Xinjiang Hui ethnic minority based on 21 STRs

  • Qiong Lan
  • Jiangang Chen
  • Yuxin Guo
  • Tong Xie
  • Yating Fang
  • Xiaoye Jin
  • Wei Cui
  • Yongsong Zhou
  • Bofeng ZhuEmail author
Original Article

Abstract

In the present study, we calculated the allelic frequencies and forensic descriptive parameters of Hui ethnic minority on the basis of 21 short tandem repeat (STR) loci aiming at understanding population structure better and enriching population genetic database. Bloodstain samples of 506 unrelated healthy Hui individuals in Xinjiang Uygur Autonomous Region were collected. Altogether 268 alleles were observed and the allelic frequencies ranged from 0.0010 to 0.5306. The combined power of discrimination and the cumulative probability of exclusion of the 21 STR loci in Hui ethnic minority were 0.9999999999999999999999998697 and 0.9999999968, respectively. Population data obtained manifested that the panel of 21 STR loci could provide robust genetic information for individual identification and paternity testing involved in forensic applications for Huis of Xinjiang Region. Furthermore, the present results of interpopulation differentiations, phylogenetic trees and principal component analysis which were conducted based on the overlapping 16 STR loci revealed that Hui group was genetically close to Xibe ethnic group and Han populations from different regions.

Keywords

Hui ethnic minority STR GlobalFiler Population differentiation 

Notes

Funding

This work was supported by the National Natural Science Foundation of China (NSFC, Nos. 81525015, 81373248), GDUPS (2017).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Flores S, Sun J, King J, Budowle B (2014) Internal validation of the GlobalFiler Express PCR Amplification Kit for the direct amplification of reference DNA samples on a high-throughput automated workflow. Forensic Sci Int Genet 10:33–39.  https://doi.org/10.1016/j.fsigen.2014.01.005 CrossRefPubMedGoogle Scholar
  2. 2.
    Ossowski A, Diepenbroek M, Szargut M, Zielinska G, Jedrzejczyk M, Berent J, Jacewicz R (2017) Population analysis and forensic evaluation of 21 autosomal loci included in GlobalFiler PCR Kit in Poland. Forensic Sci Int Genet 29:e38–e39.  https://doi.org/10.1016/j.fsigen.2017.05.003 CrossRefPubMedGoogle Scholar
  3. 3.
    Walsh PS, Metzger DA, Higushi R (2013) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10(4):506–513 (1991). Biotechniques 54(3):134–139Google Scholar
  4. 4.
    M N (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89(3):583Google Scholar
  5. 5.
    Rousset F (2008) genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Resour 8(1):103–106.  https://doi.org/10.1111/j.1471-8286.2007.01931.x CrossRefPubMedGoogle Scholar
  6. 6.
    Excoffier L, Laval G, Schneider S (2007) Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50PubMedPubMedCentralGoogle Scholar
  7. 7.
    Kovach WL (2007) MVSP - A  Multivariate Statistical Package for Windows, ver 3.1. Kovach Computing Services, PentraethGoogle Scholar
  8. 8.
    Hattemer HH (1982) Genetic distance between populations: Part 3: Wahlund’s principle as related to genetic distance and an application. TAG Theor Appl Genet 62(3):219–223.  https://doi.org/10.1007/bf00276242 PubMedGoogle Scholar
  9. 9.
    Yuan L, Ou Y, Liao Q, Gui J, Bai X, Ge J, Ye J, Zhang L (2014) Population genetics analysis of 38 STR loci in the She population from Fujian Province of China. Leg Med (Tokyo) 16(5):314–318.  https://doi.org/10.1016/j.legalmed.2014.05.008 CrossRefGoogle Scholar
  10. 10.
    Yin C, Ji Q, Li K, Mu H, Zhu B, Yan J, Yu Y, Wang J, Chen F (2015) Analysis of 19 STR loci reveals genetic characteristic of eastern Chinese Han population. Forensic Sci Int Genet 14:108–109.  https://doi.org/10.1016/j.fsigen.2014.09.015 CrossRefPubMedGoogle Scholar
  11. 11.
    Hongdan W, Bing K, Ning S, Miao H, Bo Z, Yuxin G, Bofeng Z, Shixiu L, Zhaoshu Z (2017) Evaluation of the genetic parameters and mutation analysis of 22 STR loci in the central Chinese Han population. Int J Legal Med 131(1):103–105.  https://doi.org/10.1007/s00414-016-1389-1 CrossRefPubMedGoogle Scholar
  12. 12.
    Xiuyan R, Weini W, Yaran Y, Bingbing X, Jing C, Yacheng L, Jiangwei Y (2015) Genetic variability and phylogenetic analysis of 39 short tandem repeat loci in Beijing Han population. Yi Chuan 37(7):683–691.  https://doi.org/10.16288/j.yczz.15-025 Google Scholar
  13. 13.
    Meng H, Guo Y, Dong Q, Yang G, Yan J, Shi J, Zhu B (2016) Autosomal-STR based genetic structure of Chinese Xibe ethnic group and its relationships to various groups. Int J Legal Med 130(6):1501–1503.  https://doi.org/10.1007/s00414-016-1369-5 CrossRefPubMedGoogle Scholar
  14. 14.
    Zhang H, Xia M, Qi L, Dong L, Song S, Ma T, Yang S, Jin L, Li L, Li S (2016) Forensic and population genetic analysis of Xinjiang Uyghur population on 21 short tandem repeat loci of 6-dye GlobalFiler PCR Amplification kit. Forensic Sci Int Genet 22:22–24.  https://doi.org/10.1016/j.fsigen.2016.01.005 CrossRefPubMedGoogle Scholar
  15. 15.
    Almeida C, Ribeiro T, Oliveira AR, Porto MJ, Costa Santos J, Dias D, Dario P (2015) Population data of the GlobalFiler((R)) Express loci in South Portuguese population. Forensic Sci Int Genet 19:39–41.  https://doi.org/10.1016/j.fsigen.2015.06.001 CrossRefPubMedGoogle Scholar
  16. 16.
    Hill CR, Duewer DL, Kline MC, Coble MD, Butler JM (2013) U.S. population data for 29 autosomal STR loci. Forensic Sci Int Genet 7(3):e82–e83.  https://doi.org/10.1016/j.fsigen.2012.12.004 CrossRefPubMedGoogle Scholar
  17. 17.
    Ristow PG, Cloete KW, D’Amato ME (2016) GlobalFiler((R)) Express DNA amplification kit in South Africa: extracting the past from the present. Forensic Sci Int Genet 24:194–201.  https://doi.org/10.1016/j.fsigen.2016.07.007 CrossRefPubMedGoogle Scholar
  18. 18.
    Yao J, Wang BJ (2016) Genetic variation of 25 Y-chromosomal and 15 autosomal STR loci in the Han Chinese population of Liaoning Province, Northeast China. PLoS ONE 11(8):e0160415.  https://doi.org/10.1371/journal.pone.0160415 CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155(2):945–959PubMedPubMedCentralGoogle Scholar
  20. 20.
    Rosenberg NA, Pritchard JK, Weber JL, Cann HM, Kidd KK, Zhivotovsky LA, Feldman MW (2002) Genetic structure of human populations. Science 298(5602):2381–2385.  https://doi.org/10.1126/science.1078311 CrossRefPubMedGoogle Scholar
  21. 21.
    Yang X, Zhang X, Zhu J, Chen L, Liu C, Feng X, Chen L, Wang H, Liu C (2017) Genetic analysis of 19 X chromosome STR loci for forensic purposes in four Chinese ethnic groups. Sci Rep 7:42782.  https://doi.org/10.1038/srep42782 CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Li X, Li L, Wang Q, Zhang J, Ge W, Bai R, Yu X, Shi M (2017) Population genetic analysis of the Globalfiler STR loci in 3032 individuals from the Altay Han population of Xinjiang in northwest China. Int J Legal Med.  https://doi.org/10.1007/s00414-017-1641-3 Google Scholar
  23. 23.
    Yao YG, Kong QP, Wang CY, Zhu CL, Zhang YP (2004) Different matrilineal contributions to genetic structure of ethnic groups in the silk road region in China. Mol Biol Evol 21(12):2265CrossRefPubMedGoogle Scholar
  24. 24.
    Lipman JN (1997) Familiar strangers: a history of muslims in Northwest China. Am Hist Rev 38(1):1–4Google Scholar
  25. 25.
    Shen CM, Zhu BF, Deng YJ, Ye SH, Yan JW, Yang G, Wang HD, Qin HX, Huang QZ, Zhang JJ (2010) Allele polymorphism and haplotype diversity of HLA-A, -B and -DRB1 loci in sequence-based typing for Chinese Uyghur ethnic group. PLoS ONE 5(11):e13458.  https://doi.org/10.1371/journal.pone.0013458 CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    Yan-fen S (2016) From region to entirety: a historical anthropology study on Hui Muslims’ history. J Beifang Univ Natl (4):78–81Google Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Qiong Lan
    • 1
  • Jiangang Chen
    • 2
  • Yuxin Guo
    • 3
    • 4
  • Tong Xie
    • 1
  • Yating Fang
    • 1
  • Xiaoye Jin
    • 3
    • 4
  • Wei Cui
    • 3
    • 4
  • Yongsong Zhou
    • 1
  • Bofeng Zhu
    • 1
    • 3
    • 4
    Email author
  1. 1.Department of Forensic Genetics, School of Forensic MedicineSouthern Medical UniversityGuangzhouPeople’s Republic of China
  2. 2.Institute of Forensic ScienceMinistry of Public SecurityBeijingPeople’s Republic of China
  3. 3.Key laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of StomatologyXi’an Jiaotong UniversityXi’anPeople’s Republic of China
  4. 4.Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of StomatologyXi’an Jiaotong UniversityXi’anPeople’s Republic of China

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