Genetic structure and polymorphism analysis of Xinjiang Hui ethnic minority based on 21 STRs
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In the present study, we calculated the allelic frequencies and forensic descriptive parameters of Hui ethnic minority on the basis of 21 short tandem repeat (STR) loci aiming at understanding population structure better and enriching population genetic database. Bloodstain samples of 506 unrelated healthy Hui individuals in Xinjiang Uygur Autonomous Region were collected. Altogether 268 alleles were observed and the allelic frequencies ranged from 0.0010 to 0.5306. The combined power of discrimination and the cumulative probability of exclusion of the 21 STR loci in Hui ethnic minority were 0.9999999999999999999999998697 and 0.9999999968, respectively. Population data obtained manifested that the panel of 21 STR loci could provide robust genetic information for individual identification and paternity testing involved in forensic applications for Huis of Xinjiang Region. Furthermore, the present results of interpopulation differentiations, phylogenetic trees and principal component analysis which were conducted based on the overlapping 16 STR loci revealed that Hui group was genetically close to Xibe ethnic group and Han populations from different regions.
KeywordsHui ethnic minority STR GlobalFiler Population differentiation
This work was supported by the National Natural Science Foundation of China (NSFC, Nos. 81525015, 81373248), GDUPS (2017).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 1.Flores S, Sun J, King J, Budowle B (2014) Internal validation of the GlobalFiler Express PCR Amplification Kit for the direct amplification of reference DNA samples on a high-throughput automated workflow. Forensic Sci Int Genet 10:33–39. https://doi.org/10.1016/j.fsigen.2014.01.005 CrossRefPubMedGoogle Scholar
- 2.Ossowski A, Diepenbroek M, Szargut M, Zielinska G, Jedrzejczyk M, Berent J, Jacewicz R (2017) Population analysis and forensic evaluation of 21 autosomal loci included in GlobalFiler PCR Kit in Poland. Forensic Sci Int Genet 29:e38–e39. https://doi.org/10.1016/j.fsigen.2017.05.003 CrossRefPubMedGoogle Scholar
- 3.Walsh PS, Metzger DA, Higushi R (2013) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10(4):506–513 (1991). Biotechniques 54(3):134–139Google Scholar
- 4.M N (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89(3):583Google Scholar
- 7.Kovach WL (2007) MVSP - A Multivariate Statistical Package for Windows, ver 3.1. Kovach Computing Services, PentraethGoogle Scholar
- 11.Hongdan W, Bing K, Ning S, Miao H, Bo Z, Yuxin G, Bofeng Z, Shixiu L, Zhaoshu Z (2017) Evaluation of the genetic parameters and mutation analysis of 22 STR loci in the central Chinese Han population. Int J Legal Med 131(1):103–105. https://doi.org/10.1007/s00414-016-1389-1 CrossRefPubMedGoogle Scholar
- 14.Zhang H, Xia M, Qi L, Dong L, Song S, Ma T, Yang S, Jin L, Li L, Li S (2016) Forensic and population genetic analysis of Xinjiang Uyghur population on 21 short tandem repeat loci of 6-dye GlobalFiler PCR Amplification kit. Forensic Sci Int Genet 22:22–24. https://doi.org/10.1016/j.fsigen.2016.01.005 CrossRefPubMedGoogle Scholar
- 24.Lipman JN (1997) Familiar strangers: a history of muslims in Northwest China. Am Hist Rev 38(1):1–4Google Scholar
- 25.Shen CM, Zhu BF, Deng YJ, Ye SH, Yan JW, Yang G, Wang HD, Qin HX, Huang QZ, Zhang JJ (2010) Allele polymorphism and haplotype diversity of HLA-A, -B and -DRB1 loci in sequence-based typing for Chinese Uyghur ethnic group. PLoS ONE 5(11):e13458. https://doi.org/10.1371/journal.pone.0013458 CrossRefPubMedPubMedCentralGoogle Scholar
- 26.Yan-fen S (2016) From region to entirety: a historical anthropology study on Hui Muslims’ history. J Beifang Univ Natl (4):78–81Google Scholar