Isolation and characterization of LcSAP, a Leymus chinensis gene which enhances the salinity tolerance of Saccharomyces cerevisiae
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A number of members of the SAP (“stress-associated protein”) gene family have been implicated in the plant stress response. Here, a SAP gene has been isolated using PCR RACE from the perennial grass Leymus chinensis, a species which has reputation for ecological adaptability. The 17.6 kDa LcSAP product comprised 161 residues, including both an A20 domain and an AN1 domain, a feature of type I SAPs. Using a semi-quantitative RT-PCR assay to profile its transcription, it was shown that LcSAP was more strongly transcribed in the leaf than in the root under control conditions. The level of LcSAP transcription began to rise 6 h after the plant’s exposure to 400 mM NaCl, and the abundance of transcript remained stable for at least 24 h. Exposing the plant to 100 mM Na2CO3 also induced LcSAP transcription, but the abundance of SAP transcript faded after 6 h. When LcSAP was introduced into yeast cells, the transgenic cells grew better than wild type ones when the medium contained 1.4 M NaCl. The ability of LcSAP to respond to salinity stress in yeast suggests that it also makes a contribution to the stress tolerance shown by L. chinensis.
KeywordsStress associated protein Salinity Leymus chinensis Saccharomyces cerevisiae
This work was supported by Chinese Universities Scientific Fund (2452016006, 2014YB044 and 2452016006), Specialized Research Fund for the Doctoral Program of Higher Education (20130204120018 and 20130204120038), Natural Science Basic Research Plan in Shaanxi Province of China (2013JQ3006), National Natural Science Foundation of China (31401839 and 31670295), Young Talent Funding (A314021402-1522) provided by the Institute of Soil and Water Conservation, NWAFU.
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