Molecular Biology Reports

, Volume 43, Issue 8, pp 761–766 | Cite as

Alternative splicing generates novel Fads3 transcript in mice

  • Ji Yao Zhang
  • Xia Qin
  • Hui Gyu Park
  • Ellen Kim
  • Guowen Liu
  • Kumar S. D. KothapalliEmail author
  • J. Thomas BrennaEmail author
Short Communication


Fads3 is the third member of the fatty acid desaturase gene cluster; with at least eight evolutionarily conserved alternative transcripts (AT), having no clearly established function as are known for FADS2 and FADS1. Here we present identification of a novel Fads3 transcript in mice (Fads3AT9), characterize Fads3AT9 expression in mouse tissues and evaluate correlations with metabolite profiles. Total RNA obtained from mouse tissues is reverse-transcribed into cDNA and used as template for PCR reactions. Tissue fatty acids were extracted and quantified by gas chromatography. Sequencing analysis revealed complete absence of exon 2 resulting in an open reading frame of 1239 bp, encoding a putative protein of 412 aa with loss of 37 aa compared to classical Fads3 (Fads3CS). FADS3AT9 retains all the conserved regions characteristic of front end desaturase (cytochrome b5 domain and three histidine repeats). Both Fads3CS and Fads3AT9 are ubiquitously expressed in 11 mouse tissues. Fads3AT9 abundance was greater than Fads3CS in pancreas, liver, spleen, brown adipose tissue and thymus. Fads3CS expression is low in pancreas while Fads3AT9 is over ten-fold greater abundance. The eicosanoid precursor fatty acid 20:4n − 6, the immediate desaturation product of the Fads1 coded Δ5-desaturase, was highest in pancreas where Fads3CS is low. Changes in expression patterns and fatty acid profiles suggest that Fads3AT9 may play a role in the regulation and/or biosynthesis of long chain polyunsaturated fatty acids from precursors.


Fatty acid desaturase Alternative transcript Alternative splicing Long chain polyunsaturated fatty acids 



This work was supported by NIH grant R01 AT007003 from the National Center for Complementary and Integrative Health (NCCIH) and the Office of Dietary Supplements (ODS). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIH.

Author Contributions

J.T.B., K.S.D.K., X.Q., J.Y.Z. designed research; X.Q., J.Y.Z., H.P., and E.K. executed the research; J.T. B., K.S.D.K., and G.L. contributed new reagents/analytic tools; J.T.B., K.S.D.K., X.Q., and J.Y.Z. analyzed and interpreted the data; and J.T. B., K.S.D.K., X.Q., and J.Y.Z. wrote the first draft and all authors approved the final draft.

Compliance with ethical standards

Conflict of interest

All authors declare no conflict of interest.

Supplementary material

11033_2016_4018_MOESM1_ESM.docx (548 kb)
Supplementary material 1 (DOCX 547 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Ji Yao Zhang
    • 1
  • Xia Qin
    • 1
    • 2
  • Hui Gyu Park
    • 1
  • Ellen Kim
    • 1
  • Guowen Liu
    • 2
  • Kumar S. D. Kothapalli
    • 1
    Email author
  • J. Thomas Brenna
    • 1
    Email author
  1. 1.Division of Nutritional SciencesCornell UniversityIthacaUSA
  2. 2.College of Veterinary MedicineJilin UniversityJilinChina

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