Molecular Biology Reports

, Volume 43, Issue 5, pp 427–436 | Cite as

Long noncoding RNA FGFR3-AS1 promotes osteosarcoma growth through regulating its natural antisense transcript FGFR3

  • Jiabing Sun
  • Xuming Wang
  • Chunjiang Fu
  • Xiaoyu Wang
  • Jilong Zou
  • Hanbing Hua
  • Zhenggang Bi
Original Article


Long noncoding RNAs (lncRNAs), a new class of RNAs with no protein-coding potential, have been reported to have crucial roles in the regulation of a variety of tumors. However, the functions and molecular mechanisms of lncRNAs to osteosarcoma are still largely unknown. The purpose of this study is to examine the expression, functions and molecular mechanisms of a new lncRNA FGFR3 antisense transcript 1 (FGFR3-AS1) in osteosarcoma. The expression of FGFR3-AS1 was examined by real-time quantitative PCR. The regulation of FGFR3 by FGFR3-AS1 was examined by RNase protection assay, real-time quantitative PCR, western blotting, and luciferase reporter assay. The effects of FGFR3-AS1 on osteosarcoma cell proliferation and cell cycle were determined by Cell Counting Kit-8, Ethynyl deoxyuridine incorporation assay and flow cytometry. FGFR3-AS1 was upregulated in osteosarcoma. Increased FGFR3-AS1 expression correlates with large tumor size, advanced Enneking stage, metastasis and poor survival. Through antisense pairing with FGFR3 3′UTR, FGFR3-AS1 increases FGFR3 mRNA stability and upregulates FGFR3 expression. The expression of FGFR3-AS1 and FGFR3 is positively correlated in osteosarcoma tissues. Knockdown of FGFR3-AS1 inhibits the proliferation and cell cycle progression of osteosarcoma cells in vitro. Moreover, knockdown of FGFR3-AS1 inhibits xenograft tumor growth of osteosarcoma cells in vivo. These data demonstrate the mechanisms of how antisense noncoding RNA regulate the expression of sense genes, and show the pivotal functions of FGFR3-AS1 in osteosarcoma.


Osteosarcoma Long noncoding RNA Antisense transcript Proliferation 


Compliance with ethical standards

Conflicts of interest

There is no conflict of interest.


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Jiabing Sun
    • 1
  • Xuming Wang
    • 1
  • Chunjiang Fu
    • 1
  • Xiaoyu Wang
    • 1
  • Jilong Zou
    • 1
  • Hanbing Hua
    • 1
  • Zhenggang Bi
    • 1
  1. 1.Department of OrthopedicsThe First Affiliated Hospital of Harbin Medical UniversityHarbinChina

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