Identification of learning and memory genes in canine; promoter investigation and determining the selective pressure
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One of the important behaviors of dogs is trainability which is affected by learning and memory genes. These kinds of the genes have not yet been identified in dogs. In the current research, these genes were found in animal models by mining the biological data and scientific literatures. The proteins of these genes were obtained from the UniProt database in dogs and humans. Not all homologous proteins perform similar functions, thus comparison of these proteins was studied in terms of protein families, domains, biological processes, molecular functions, and cellular location of metabolic pathways in Interpro, KEGG, Quick Go and Psort databases. The results showed that some of these proteins have the same performance in the rat or mouse, dog, and human. It is anticipated that the protein of these genes may be effective in learning and memory in dogs. Then, the expression pattern of the recognized genes was investigated in the dog hippocampus using the existing information in the GEO profile. The results showed that BDNF, TAC1 and CCK genes are expressed in the dog hippocampus, therefore, these genes could be strong candidates associated with learning and memory in dogs. Subsequently, due to the importance of the promoter regions in gene function, this region was investigated in the above genes. Analysis of the promoter indicated that the HNF-4 site of BDNF gene and the transcription start site of CCK gene is exposed to methylation. Phylogenetic analysis of protein sequences of these genes showed high similarity in each of these three genes among the studied species. The dN/dS ratio for BDNF, TAC1 and CCK genes indicates a purifying selection during the evolution of the genes.
KeywordsLearning and memory genes Dog Bioinformatics
We would like to thank Dr. Mohammad Sadegh Sabet for his helpful comments during the research. This work was supported by a research grant of the Research Center of Anti narcotic Police of Iran.
Conflict of interest
There is no conflict of interest existing.
- 2.Alberini CM (2009) Transcription factors in long-term memory and synaptic plasticity. APS 89(121):121–145Google Scholar
- 3.Ardalan A, Kluetsch CFC, Zhang A, Erdogan M, Uhlén M, Houshmand M, Tepeli C, Ashtiani SRM, Savolainen P (2011) Comprehensive study of mtDNA among Southwest Asian dogs contradicts independent domestication of wolf, but implies dog–wolf hybridization. Ecol Evol 1(3):373–385Google Scholar
- 7.Hart DL, Jones EW (2005) Genetics: analysis of genes and genomes, 6th edn. Jones & Bartlett, Missisauga CanadaGoogle Scholar
- 11.Kirkness EF, Bafna V, Halpern AL, Levy S, Remington K, Rusch DB, Delcher AL, Pop M, Landsberg GM (1991) The distribution of canine behavior cases at three behavior referral practices. Vet med-us 86:1011–1018Google Scholar
- 13.Liggins JTP (2009) The roles of dopamine D1 and D2 receptors in working memory function. Msurj 4(1):39–45Google Scholar
- 17.Mineur YS, Crusio WE, Sluyter F (2004) Genetic dissection of learning and memory in mice. Neural plast 11:3–4Google Scholar
- 18.Pang JF, Kluetsch C, Zou XJ, Zhang AB, Luo LY, Angleby H, Ardalan A, Ekstrom C, Skollermo A, Lundeberg J, Matsumura S, Leitner T, Zhang YP, Savolainen P (2009) mtDNA data indicate a single origin for dogs south of Yangtze River, less than 16,300 years ago, from numerous wolves. Mol Boil Evol 26:2849–2864CrossRefGoogle Scholar
- 26.Trut LN, Kharlamova AV, Kukekova AV, Acland GM, Carrier DR, Chaes K, Lark KG (2006) Morphology and behavior: are they coupled at the genome level? In: Ostrander EA, Giger U, Lindblad-Toh K (eds) The dog and its genome. Cold Harbor Springs Laboratory Press, Cold Harbor Springs, New YorkGoogle Scholar
- 29.Vlahopoulos S, Zoumpourlis VC (2004) JNK: a key modulator of intracellular signaling. Biochemistry-us 69(8):844–854Google Scholar