Protective effect of myokine IL-15 against H2O2-mediated oxidative stress in skeletal muscle cells
The production of reactive oxygen species (ROS) during oxidative stress may cause cellular injury. Interleukin-15 (IL-15) is one of the skeletal muscle secreted myokines, and there is no information that reported its anti-oxidative capability in skeletal muscle. The aim of this study therefore is to investigate the protective effects of myokine IL-15 against H2O2-mediated oxidative stress in C2C12 myoblasts. The results showed that IL-15 pre-incubation reduced the intracellular creatine kinase and lactate dehydrogenase activities, decreased the ROS overload, and protect the mitochondrial network via up-regulated mRNA expression levels of IL-15 and uncoupling protein 3. It also down-regulated the levels of IL-6 and p21 of the myoblasts compared to the cells treated only with H2O2. Meanwhile, apurinic/aprimidinic endonuclease 1 expression and the Akt signaling pathway were stimulated. These effects could contribute to the resumption of cell viability and act as protective mechanism. In conclusion, myokine IL-15 could be a novel endogenous regulator to control intracellular ROS production and attenuate oxidative stress in skeletal muscle cells.
KeywordsIL-15 Oxidative stress H2O2 C2C12 cells
Apurinic/aprimidinic endonuclease 1
Dulbecco’s modified Eagle’s medium
Extracellular-signal regulated kinase-1/2
Myogenic differentiation antigen
Phosphate buffered saline
Reactive oxygen species
Uncoupling protein 3
This study was jointly supported by National Basic Research Program of China (2013CB127305, 2012CB124704), National Nature Science Foundation of China (31372325, 31110103909), and the Project of Institute of Subtropical Agriculture, the Chinese Academy of Sciences (ISACX-LYQY-QN-1104). The authors’ contributions were as follows: F. N. Li and Y. L. Yin were in charge of the whole trial. F. N. Li wrote the manuscript while A.O Oso assisted in technical editing and correction of the paper. Y. H. Li, Y. L. Tan and B. B. Lin assisted with the cell culture. X. F. Kong assisted with the biochemical analyses.
Conflict of interest
The authors have declared that no competing interests exist.
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