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Molecular Biology Reports

, Volume 41, Issue 11, pp 7715–7722 | Cite as

Protective effect of myokine IL-15 against H2O2-mediated oxidative stress in skeletal muscle cells

  • Fengna Li
  • Yinghui Li
  • Yulong Tang
  • Binbin Lin
  • Xiangfeng Kong
  • Oso Abimbola Oladele
  • Yulong YinEmail author
Article

Abstract

The production of reactive oxygen species (ROS) during oxidative stress may cause cellular injury. Interleukin-15 (IL-15) is one of the skeletal muscle secreted myokines, and there is no information that reported its anti-oxidative capability in skeletal muscle. The aim of this study therefore is to investigate the protective effects of myokine IL-15 against H2O2-mediated oxidative stress in C2C12 myoblasts. The results showed that IL-15 pre-incubation reduced the intracellular creatine kinase and lactate dehydrogenase activities, decreased the ROS overload, and protect the mitochondrial network via up-regulated mRNA expression levels of IL-15 and uncoupling protein 3. It also down-regulated the levels of IL-6 and p21 of the myoblasts compared to the cells treated only with H2O2. Meanwhile, apurinic/aprimidinic endonuclease 1 expression and the Akt signaling pathway were stimulated. These effects could contribute to the resumption of cell viability and act as protective mechanism. In conclusion, myokine IL-15 could be a novel endogenous regulator to control intracellular ROS production and attenuate oxidative stress in skeletal muscle cells.

Keywords

IL-15 Oxidative stress H2O2 C2C12 cells 

Abbreviations

APE1

Apurinic/aprimidinic endonuclease 1

CK

Creatin kinase

DMEM

Dulbecco’s modified Eagle’s medium

ERK1/2

Extracellular-signal regulated kinase-1/2

LDH

Lactate dehydrogenase

MyoD

Myogenic differentiation antigen

PBS

Phosphate buffered saline

ROS

Reactive oxygen species

SOD

Superoxide dismutase

UCP3

Uncoupling protein 3

Notes

Acknowledgments

This study was jointly supported by National Basic Research Program of China (2013CB127305, 2012CB124704), National Nature Science Foundation of China (31372325, 31110103909), and the Project of Institute of Subtropical Agriculture, the Chinese Academy of Sciences (ISACX-LYQY-QN-1104). The authors’ contributions were as follows: F. N. Li and Y. L. Yin were in charge of the whole trial. F. N. Li wrote the manuscript while A.O Oso assisted in technical editing and correction of the paper. Y. H. Li, Y. L. Tan and B. B. Lin assisted with the cell culture. X. F. Kong assisted with the biochemical analyses.

Conflict of interest

The authors have declared that no competing interests exist.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Fengna Li
    • 1
  • Yinghui Li
    • 1
    • 2
  • Yulong Tang
    • 1
  • Binbin Lin
    • 1
    • 3
  • Xiangfeng Kong
    • 1
  • Oso Abimbola Oladele
    • 4
  • Yulong Yin
    • 1
    Email author
  1. 1.Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical AgricultureChinese Academy of SciencesChangshaChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.College of Animal SciencesHunan Agricultural UniversityChangshaChina
  4. 4.Animal Nutrition Department, College of Animal Science and Livestock ProductionFederal University of Agriculture AbeokutaAbeokutaNigeria

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