Molecular Biology Reports

, Volume 41, Issue 11, pp 7141–7151 | Cite as

Associations of platelet-activating factor acetylhydrolase (PAF-AH) gene polymorphisms with circulating PAF-AH levels and risk of coronary heart disease or blood stasis syndrome in the Chinese Han population

  • Guo-Hua Zheng
  • Shang-Quan Xiong
  • Hai-Ying Chen
  • Li-Juan Mei
  • Ting Wang
Article

Abstract

The circulating level of platelet-activating factor acetylhydrolase (PAF-AH) is a novel biomarker to predict the presence of coronary heart disease. PAF-AH gene polymorphisms may be responsible for the variance of circulating PAF-AH levels in individuals. However, the association of PAF-AH gene polymorphisms with circulating PAF-AH levels and the susceptibility to coronary heart disease (CHD) remains unsolved. Blood stasis syndrome (BSS) of CHD is the most common type of TCM syndromes, and a previous study discovered its relationship with the elevated circulating PAF-AH levels. However, the association of gene polymorphisms and CHD with BSS is unclear at present. In this study, four polymorphisms (R92H, I198T, A379V, V279F) of the PAF-AH gene were genotyped in 570 CHD patients, of which 299 had BSS. In addition, 317 unaffected individuals from the same hospitals served as controls. Plasma PAF-AH levels were measured in 155 controls and 271 CHD patients selected randomly, including 139 CHD patients with BSS. In the Chinese Han population, plasma PAF-AH levels in CHD patients with BSS or without BSS were significantly higher (12.9 ± 6.5 and 11.1 ± 5.0 μM, respectively) than in controls (9.3 ± 5.2 μM); this difference still remained significant after adjustment for traditional risk factors or the inflammatory factors. The R92H polymorphism was highly related to the plasma PAF-AH levels and the risk of CHD, especially among patients with BSS, even with the adjustment for the effects of traditional factors. The I198T polymorphism was highly associated with risk of CHD with BSS, but was associated with neither the risk of CHD with no BSS nor with elevated plasma PAF-AH levels.

Keywords

Platelet-activating factor acetylhydrolase Gene polymorphism Coronary heart disease Blood stasis syndrome Association 

Abbreviations and acronyms

BMI

Body mass index

BSS

Blood stasis syndrome

CHD

Coronary heart disease

95 % Cl

95 % Confidence interval

dNTPs

Deoxyribonucleotide triphosphates

ECG

Electrocardiograph

GWAS

Genome-wide association study

HDL-C

High density lipoprotein cholesterol

HWE

Hardy–weinberg equilibrium

hs-CRP

High sensitivity C-reaction protein

IL-6

Interleukin 6

LD

Linkage disequilibrium

LDL-C

Low density lipoprotein cholesterol

LP-PLA2

Lipoprotein-associated phospholipase A2

MAF

The minor allele frequency

OR

Odds ratio

PAF-AH

Platelet activating factor acetylhydrolase

PCR

Polymerase chain reaction

SAP

Shrimp alkaline phosphatase

SD

Standard deviation

SNP

Single nucleotide polymorphism

TC

Total cholesterol

TCM

Traditional Chinese medicine

TG

Triglycerides

Notes

Acknowledgments

Sources of Funding: This study was supported by research funds from the National Natural Science Foundation of China (Grant No: 81072726, National Natural Science Foundation of China, Beijin, China) and the Natural Science Foundation of Fujian province (Grant No: 2009J01163, Fujian Provincial Department of Science & Technology, Fujian, China). But the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Conflict of interest

None.

Supplementary material

11033_2014_3597_MOESM1_ESM.doc (72 kb)
Supplementary material 1 (DOC 72 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Guo-Hua Zheng
    • 1
  • Shang-Quan Xiong
    • 2
  • Hai-Ying Chen
    • 1
  • Li-Juan Mei
    • 1
  • Ting Wang
    • 2
  1. 1.College of Rehabilitation MedicineFujian University of Traditional Chinese MedicineFuzhouChina
  2. 2.Department of Cardiologic MedicineThe People’s Hospital of Fujian ProvinceFuzhouChina

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