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Molecular Biology Reports

, Volume 41, Issue 9, pp 6215–6223 | Cite as

NDRG1 expression is related to the progression and prognosis of gastric cancer patients through modulating proliferation, invasion and cell cycle of gastric cancer cells

  • Xiaojing Chang
  • Xiaoyang Xu
  • Jinguo Ma
  • Xiaoying Xue
  • Zhenhua Li
  • Peng Deng
  • Shuanglong Zhang
  • Yu Zhi
  • Jing Chen
  • Dongqiu DaiEmail author
Article

Abstract

N-myc downstream-regulated gene 1 (NDRG1) has been proposed as a tumor suppressor gene in many different types of tumors, but its potential function and corresponding mechanism are not yet fully elucidated. This study aims to detect the possible function of NDRG1 in gastric cancer progression. In this study, 112 paired gastric cancer tissues and corresponding nonmalignant gastric tissues were utilized to identify the differential protein expression of NDRG1 by immunohistochemistry and its clinical significance was analyzed. Furthermore, 49 of 112 paired gastric specimens were used to detect the differential mRNA expression by real-time PCR. The over expression of NDRG1 in human gastric cancer cell line AGS by PcDNA3.1–NDRG1 transfection was utilized to detect the role of NDRG1 in regulating the biological behavior of gastric cancer. NDRG1 expression was significantly decreased in primary gastric cancer tissues, compared with its corresponding nonmalignant gastric tissues (p < 0.05), and its decreased expression was significantly associated with lymph node metastasis (p < 0.01), invasion depth (p < 0.01) and differentiation (p < 0.05). Additionally, the overall survival rate of gastric cancer patients with high expression of NDRG1 was higher than those with low expression during the follow-up period. NDRG1 overexpression suppressed cells proliferation, invasion and induced a G1 cell cycle arrest in gastric cancer. Furthermore, the down-regulation of NDRG1 in gastric cancer metastatic progression was correlated to E-cadherin and MMP-9. Our results verify that NDRG1 acts as a tumor suppressor gene and may play an important role in the metastasis progression and prognosis of gastric cancer.

Keywords

NDRG1 Differentiation-related gene Gastric cancer Metastasis 

Notes

Acknowledgments

This work was supported in part by a grant from the National Natural Science Foundation of China (#30572162), the Higher Specialized Research Fund for Doctoral Program of Ministry of Education of China (#20102104110001) and the Liaoning Province Science and Technology Plan Project (#2013225021). The authors would like to thank Institute of General Surgery of China Medical University for supports.

Conflict of interest

No conflicts of interest are declared by the authors.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Xiaojing Chang
    • 1
    • 2
  • Xiaoyang Xu
    • 1
  • Jinguo Ma
    • 1
  • Xiaoying Xue
    • 3
  • Zhenhua Li
    • 1
  • Peng Deng
    • 1
  • Shuanglong Zhang
    • 1
  • Yu Zhi
    • 1
  • Jing Chen
    • 1
  • Dongqiu Dai
    • 1
    • 2
    Email author
  1. 1.Department of Gastrointestinal Surgery, The Fourth Affiliated HospitalChina Medical UniversityShenyangChina
  2. 2.Cancer Center, The Fourth Affiliated HospitalChina Medical UniversityShenyangChina
  3. 3.Department of Radiotherapy, The Second HospitalHebei Medical UniversityShijiazhuangChina

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