Molecular Biology Reports

, Volume 41, Issue 8, pp 5413–5427 | Cite as

Molecular characterization and expressional affirmation of the beta proteasome subunit cluster in rock bream immune defense

  • Saranya Revathy Kasthuri
  • Navaneethaiyer Umasuthan
  • Ilson Whang
  • Bong-Soo Lim
  • Hyung-Bok Jung
  • Myung-Joo Oh
  • Sung-Ju Jung
  • Sang-Yeob Yeo
  • Sung Yeon Kim
  • Jehee Lee
Article

Abstract

Immunoproteasomes are primarily induced upon infection and formed by replacing constitutive beta subunits with inducible beta subunits which possess specific cleavage properties that aid in the release of peptides necessary for MHC class I antigen presentation. In this study, we report the molecular characterization and expression analysis of the inducible immunosubunits PSMB8, PSMB9, PSMB9-L, and PSMB10 from rock bream, Oplegnathus fasciatus. The three subunits shared common active site residues and were placed in close proximity to fish homologues in the reconstructed phylogenetic tree, in which the mammalian homologues formed separate clades, indicating a common ancestral origin. The rock bream immunosubunits possessed higher identity and similarity with the fish homologues. RbPSMB8, RbPSMB9, RbPSMB9-L, and RbPSMB10 were multi-exonic genes with 6, 6, 7 and 8 exons, respectively. These four genes were constitutively expressed in all the examined tissues. Immunostimulants such as lipopolysaccharide and poly I:C induced RbPSMB8, RbPSMB9, RbPSMB9-L, and RbPSMB10 in liver and head kidney, suggesting their possible involvement in immune defense in rock bream.

Keywords

Immunoproteasome Low molecular weight protein 7 Low molecular weight protein 2 Low molecular weight protein 2-like Multi-catalytic endopeptidase complex-like 1 

Notes

Acknowledgments

This study was supported by a National Fisheries Research and Development Institute (RP-2014-BT-011) Grant.

Supplementary material

11033_2014_3413_MOESM1_ESM.doc (173 kb)
Supplementary material 1 (DOC 173 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Saranya Revathy Kasthuri
    • 1
  • Navaneethaiyer Umasuthan
    • 1
    • 2
  • Ilson Whang
    • 1
    • 2
  • Bong-Soo Lim
    • 2
  • Hyung-Bok Jung
    • 2
  • Myung-Joo Oh
    • 3
  • Sung-Ju Jung
    • 3
  • Sang-Yeob Yeo
    • 4
  • Sung Yeon Kim
    • 5
  • Jehee Lee
    • 1
    • 2
  1. 1.Department of Marine Life Sciences, School of Marine Biomedical SciencesJeju National UniversityJejuRepublic of Korea
  2. 2.Fish Vaccine Development CenterJeju National UniversityJejuRepublic of Korea
  3. 3.Department of Aqualife MedicineChonnam National UniversityChonnamRepublic of Korea
  4. 4.Division of Applied Chemistry & Biotechnology, Department of BiotechnologyHanbat National UniversityDaejeonRepublic of Korea
  5. 5.Genetics & Breeding Research CenterNational Fisheries Research & Development InstituteGeojeRepublic of Korea

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