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Molecular Biology Reports

, Volume 41, Issue 8, pp 5321–5327 | Cite as

The role of bronchial epithelial cell apoptosis in the pathogenesis of COPD

  • B. GogebakanEmail author
  • R. Bayraktar
  • M. Ulaslı
  • S. Oztuzcu
  • D. Tasdemir
  • H. Bayram
Article

Abstract

There is an increased airway inflammation in the pathogenesis of chronic obstructive pulmonary disease (COPD), and it has been suggested that there may also be problem in the apoptosis and renewal of cells. However, there are limited human airway cell studies, in particular those from larger airways such as bronchi. We cultured primary human bronchial epithelial cells (HBECs) from bronchial explants of smokers (n = 6) without COPD and smokers with COPD (n = 8). Apoptosis was studied by fluorescence activated cell sorting. qRT-PCR was used to assess mRNA expression for proteins involving apoptosis including p21CIP1/WAF1, p53, caspase-8 and caspase-9. Although there was no difference in the rate of viable cells between cells from smokers and COPDs, the level of early apoptotic cells was significantly increased in COPD cells [mean ± standard error of mean (SEM) = 4.86 ± 3.2 %, p = 0.015] as compared to smokers (mean ± SEM = 2.71 ± 1.62 %). In contrast, the rate of late apoptotic cells was significantly decreased in COPD cells (mean ± SEM = 9.82 ± 5.71 %) comparing to smokers (mean ± SEM = 15.21 ± 5.08 %, p = 0.003). Although expression of mRNA for p21CIP1/WAF1 and caspase-9 was similar in both groups, p53 and caspase-8 mRNA expression was significantly greater in COPD cells. These findings suggest that HBEC apoptosis is increased in COPD, and that this involves p53 and caspase-8 pathways.

Keywords

Apoptosis Caspases COPD Primary bronchial epithelial cells p21 p53 

Notes

Acknowledgments

This study is funded by The Research Fund of Gaziantep University with the Grant No.: TF.11.32.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • B. Gogebakan
    • 1
    • 3
    Email author
  • R. Bayraktar
    • 2
  • M. Ulaslı
    • 2
  • S. Oztuzcu
    • 2
  • D. Tasdemir
    • 3
  • H. Bayram
    • 3
  1. 1.Department of Medical Biology, Faculty of Medicine, School of MedicineMustafa Kemal UniversityHatayTurkey
  2. 2.Department of Medical Biology, School of MedicineUniversity of GaziantepGaziantepTurkey
  3. 3.Respiratory Research Laboratory, Department of Respiratory Medicine, School of MedicineUniversity of GaziantepGaziantepTurkey

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