Isolation and characterization of a pathogenesis-related protein 10 gene (GmPR10) with induced expression in soybean (Glycine max) during infection with Phytophthora sojae
- 991 Downloads
In previous study, a cDNA library enriched for mRNAs encoding ESTs that increased in abundance during infection with Phytophthora sojae was constructed by suppression subtractive hybridization from leaf tissues of a high resistant soybean, and an EST homologous to the class 10 of pathogenesis-related (PR) proteins was identified to be up-regulated by microarray and real-time PCR. Here, the full-length cDNA (termed GmPR10, GenBank accession number FJ960440; ADC31789.1) of the EST was isolated by rapid amplification of cDNA ends, and contains an open reading frame of 474 bp. The GmPR10 protein included a “P-loop’’ motif. The constitutive transcript abundance of GmPR10 in soybean was the highest in leaves, followed by roots and stems. Further analysis showed that GmPR10 mRNA abundance was increased during infection with P. sojae following leaf treatments with gibberellin (GA3), hydrogen peroxide (H2O2), salicylic acid (SA), and abscisic acid (ABA). The dialytically renatured GmPR10 protein significantly inhibited P. sojae hyphal growth and exhibited RNase activity. Transgenic tobacco and soybean plants overexpressing GmPR10 showed increased resistance to P. nicotianae Breda and P. sojae, respectively. These results suggest that the GmPR10 protein plays an important role in host defense against P. sojae infection. To the best of our knowledge, this is the first report on the functional characterization of a PR10 protein from soybean in defense against P. sojae.
KeywordsPathogenesis-related (PR) proteins Soybean (Glycine max) Phytophthora sojae PR10 gene
The research was supported through funding from the Heilongjiang Province outstanding youth fund (JC201308), NSFC Projects (31071439, 31171577, 31101167), the Specialized Research Fund for the Doctoral Program of Higher Education (20112325120005), the Science and Technology Innovation Project in Harbin (2012RFQXN011, 2012RFXXN019), and the Research Fund for Young Teachers through NEAU (2012 RCB 08).
- 1.Anderson TR, Tenuta A (2003) Phytophthora rot. In: Bailey KL, Gossen BD, Gossen BD, Gugel RK, Morrall RAA (eds) Diseases of field crops in Canada. The Canadian Phytopathological Society, Saskatoon, pp 155–156Google Scholar
- 4.Bantignies B, Seguin J et al (2000) Direct evidence for ribonucleolytic activity of a PR-10-like protein from white lupin roots. Plant Mol Biol 871:842–881Google Scholar
- 13.Dubods C, Plomion C (2001) Drought differentially affect expression of a PR10 protein, in needles of maritime pine (Pinus pinaster Ait.) seedlings. J Exp Bot 1143:1144–1152Google Scholar
- 14.Dou DL, Wang BS et al (2003) Transgenic tobacco with NDR1 gene improved its resistance to two fungal diseases. Sci Agric Sin 36:1120–1124Google Scholar
- 16.Edreva A (2005) Pathogenesis-related proteins: research progress in the last 15 years. Gen Appl Plant Physiol 31:105–124Google Scholar
- 17.Erwin DC, Ribeiro OK (1996) Phytophthora Diseases Worldwide. APS, St. Paul, p 592Google Scholar
- 27.Kaufmann MJ, Gerdemann JW (1958) Root and stem rot of soybean caused by Phytophthora sojae n. sp. Phytopathology 48:201–208Google Scholar
- 47.Rakwal R, Agrawal GK et al (1999) Separation of proteins from stressed rice Oryza sativa L. leaf tissues by two dimensional polyacrylamide gel electrophoresis, induction of pathogenesis-related and cellular protectant proteins by jasmonic acid, UV irradiation and copper chloride. Electrophoresis 20:3472–3478CrossRefPubMedGoogle Scholar
- 49.Sambrook J, Fritsch EF et al (1989) In molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, pp 9–62Google Scholar