Molecular Biology Reports

, Volume 41, Issue 7, pp 4623–4629 | Cite as

Differential regulation of microRNAs in response to osmotic, salt and cold stresses in wheat

  • Om Prakash Gupta
  • Nand Lal Meena
  • Indu Sharma
  • Pradeep Sharma
Article

Abstract

MicroRNAs (miRNAs) are tiny non-coding regulatory molecules that modulate plant’s gene expression either by cleaving or repressing their mRNA targets. To unravel the plant actions in response to various environmental factors, identification of stress related miRNAs is essential. For understanding the regulatory behaviour of various abiotic stresses and miRNAs in wheat genotype C-306, we examined expression profile of selected conserved miRNAs viz. miR159, miR164, miR168, miR172, miR393, miR397, miR529 and miR1029 tangled in adapting osmotic, salt and cold stresses. The investigation revealed that two miRNAs (miR168, miR397) were down-regulated and miR172 was up-regulated under all the stress conditions. However, miR164 and miR1029 were up-regulated under cold and osmotic stresses in contrast to salt stress. miR529 responded to cold alone and does not change under osmotic and salt stress. miR393 showed up-regulation under osmotic and salt, and down-regulation under cold stress indicating auxin based differential cold response. Variation in expression level of studied miRNAs in presence of target genes delivers a likely elucidation of miRNAs based abiotic stress regulation. In addition, we reported new stress induced miRNAs Ta-miR855 using computational approach. Results revealed first documentation that miR855 is regulated by salinity stress in wheat. These findings indicate that diverse miRNAs were responsive to osmotic, salt and cold stress and could function in wheat response to abiotic stresses.

Keywords

miRNAs Wheat Drought Cold Salt 

Supplementary material

11033_2014_3333_MOESM1_ESM.doc (34 kb)
Supplementary material 1 (DOC 33 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Om Prakash Gupta
    • 1
  • Nand Lal Meena
    • 1
    • 2
    • 3
  • Indu Sharma
    • 3
  • Pradeep Sharma
    • 3
  1. 1.Quality and Basic Sciences DivisionDirectorate of Wheat ResearchKarnalIndia
  2. 2.Basic Sciences DivisionIndian Institute of Pulses ResearchKanpurIndia
  3. 3.Biotechnology LaboratoryDirectorate of Wheat ResearchKarnalIndia

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