Genetic analysis of an F2 intercross between two strains of Japanese quail provided evidence for quantitative trait loci affecting carcass composition and internal organs
- 133 Downloads
The purpose of this study was to identify genomic regions, quantitative trait loci (QTL), affecting carcass traits on chromosome 1 in an F2 population of Japanese quail. For this purpose, two white and wild strains of Japanese quail (16 birds) were crossed reciprocally and F1 generation (34 birds) was created. The F2 generation was produced by intercrossing of the F1 birds. Phenotypic data including carcass weight, internal organs and carcass parts were collected on F2 animals (422 birds). The total mapping population (472 birds) was genotyped for 8 microsatellite markers on chromosome 1. QTL analysis was performed with interval mapping method applying the line-cross model. Significant QTL were identified for breast weight at 0 (P < 0.01), 172 (P < 0.05) and 206 (P < 0.01), carcass weight at 91 (P < 0.05), carcass fatness at 0 (P < 0.01), pre-stomach weight at 206 (P < 0.01) and uropygial weight gland at 197 (P < 0.01) cM on chromosome 1. There was also evidence for imprinted QTL affecting breast weight (P < 0.01) on chromosome 1. The proportion of the F2 phenotypic variation explained by the significant additive, dominance and imprinted QTL effects ranged from 1.0 to 7.3 %, 1.2 to 3.3 % and 1.4 to 2.2 %, respectively.
KeywordsCarcass traits Coturnix japonica F2 design Microsatellite markers QTL mapping
This research was funded by the Institute of Science, High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran (Research project number 1/1142). The authors are grateful to the staff of the Livestock Research Centre and students in the Department of Animal Science at Shahid Bahonar University of Kerman for their help in collecting the phenotypic data and blood samples.
- 1.Minvielle F, Kayang BB, Inoue-Murayama M, Miwa M, Vignal A, Gourichon D, Neau A, Monvoisin JL, Ito S (2005) Microsatellite mapping of QTL affecting growth, feed consumption, egg production, tonic immobility and body temperature of Japanese quail. BMC Genomics 6:87PubMedCentralCrossRefPubMedGoogle Scholar
- 15.Tercic D, Holcman A, Dovc P, Morrice DR, Burt DW, Hocking PM, Horvat S (2009) Identification of chromosomal regions associated with growth and carcass traits in an F3 full sib intercross line originating from a cross of chicken lines divergently selected on body weight. Anim Genet 40:743–748CrossRefPubMedGoogle Scholar
- 23.Gilmour AR, Gogel BJ, Cullis BR, Thompson R (2006) Asreml [computer program]. Version 2.0. VSN International Ltd, Hemel HempsteadGoogle Scholar
- 29.Nadaf J, Pitel F, Gilbert H, Duclos MJ, Vignoles F, Beaumont C, Vignal A, Porter TE, Cogburn LA, Aggrey SE, Simon J, Le Bihan-Duval E (2009) QTL for several metabolic traits map to loci controlling growth and body composition in an F2 intercross between high- and low-growth chicken lines. Physiol Genomics 38:241–249CrossRefPubMedGoogle Scholar
- 34.Sohrabi S, Esmailizadeh AK, Moradian H (2011) Study of the reciprocal cross effects on carcass traits in the F2 birds from an intercross between two strains in Japanese quail. The First National Seminar of Animal Production in the Hot Climates, Kerman, pp 1486–1489Google Scholar