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Molecular Biology Reports

, Volume 41, Issue 7, pp 4455–4462 | Cite as

Genetic analysis of an F2 intercross between two strains of Japanese quail provided evidence for quantitative trait loci affecting carcass composition and internal organs

  • Hasan Moradian
  • Ali K. Esmailizadeh
  • Saeed S. Sohrabi
  • Ehsan Nasirifar
  • Nahid Askari
  • Mohammad Reza Mohammadabadi
  • Amin Baghizadeh
Article

Abstract

The purpose of this study was to identify genomic regions, quantitative trait loci (QTL), affecting carcass traits on chromosome 1 in an F2 population of Japanese quail. For this purpose, two white and wild strains of Japanese quail (16 birds) were crossed reciprocally and F1 generation (34 birds) was created. The F2 generation was produced by intercrossing of the F1 birds. Phenotypic data including carcass weight, internal organs and carcass parts were collected on F2 animals (422 birds). The total mapping population (472 birds) was genotyped for 8 microsatellite markers on chromosome 1. QTL analysis was performed with interval mapping method applying the line-cross model. Significant QTL were identified for breast weight at 0 (P < 0.01), 172 (P < 0.05) and 206 (P < 0.01), carcass weight at 91 (P < 0.05), carcass fatness at 0 (P < 0.01), pre-stomach weight at 206 (P < 0.01) and uropygial weight gland at 197 (P < 0.01) cM on chromosome 1. There was also evidence for imprinted QTL affecting breast weight (P < 0.01) on chromosome 1. The proportion of the F2 phenotypic variation explained by the significant additive, dominance and imprinted QTL effects ranged from 1.0 to 7.3 %, 1.2 to 3.3 % and 1.4 to 2.2 %, respectively.

Keywords

Carcass traits Coturnix japonica F2 design Microsatellite markers QTL mapping 

Notes

Acknowledgments

This research was funded by the Institute of Science, High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran (Research project number 1/1142). The authors are grateful to the staff of the Livestock Research Centre and students in the Department of Animal Science at Shahid Bahonar University of Kerman for their help in collecting the phenotypic data and blood samples.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Hasan Moradian
    • 1
  • Ali K. Esmailizadeh
    • 1
  • Saeed S. Sohrabi
    • 1
  • Ehsan Nasirifar
    • 2
  • Nahid Askari
    • 3
  • Mohammad Reza Mohammadabadi
    • 1
  • Amin Baghizadeh
    • 3
  1. 1.Department of Animal Science, Faculty of AgricultureShahid Bahonar University of KermanKermanIran
  2. 2.Department of Animal Science, Science and Research BranchIslamic Azad UniversityTehranIran
  3. 3.Department of Biotechnology, Institute of Science, High Technology and Environmental SciencesGraduate University of Advanced TechnologyKermanIran

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