Molecular Biology Reports

, Volume 41, Issue 6, pp 3925–3933 | Cite as

RETRACTED ARTICLE: Role of RASSF1A promoter methylation in the pathogenesis of hepatocellular carcinoma: a meta-analysis of 21 cohort studies

  • Yong-Shuang LiEmail author
  • Qiang Xie
  • Da-Ye Yang
  • Yuan Zheng


We carried out the current meta-analysis aiming to comprehensively assess the potential role of RASSF1A aberrant promoter methylation in the pathogenesis of hepatocellular carcinoma (HCC). A range of electronic databases were searched: Web of Science (1945–2013), the Cochrane Library Database (Issue 12, 2013), PubMed (1966–2013), EMBASE (1980–2013), CINAHL (1982–2013) and the Chinese Biomedical Database (CBM) (1982–2013) without language restrictions. Meta-analysis was conducted using the STATA 12.0 software. Crude risk difference (RD) with their 95 % confidence interval (95 % CI) was calculated. In the present meta-analysis, 21 clinical cohort studies with a total of 1,205 HCC patients were included. The results of our meta-analysis illustrated that the frequency of RASSF1A promoter methylation in cancer tissues were significantly higher than those of normal, adjacent and benign tissues (cancer tissues vs. normal tissues: RD = 0.63, 95 % CI 0.53–0.73, P < 0.001; cancer tissues vs. adjacent tissues: RD = 0.43, 95 % CI 0.33–0.53, P < 0.001; cancer tissues vs. benign tissues: RD = 0.48, 95 % CI 038–0.58, P < 0.001; respectively). Further subgroup by ethnicity demonstrated that RASSF1A aberrant promoter methylation was correlated with the pathogenesis of HCC among both Asians and Caucasians (all P < 0.05). The current meta-analysis suggests that RASSF1A aberrant promoter methylation may be implicated in the pathogenesis of HCC. Thus, detection of RASSF1A promoter methylation may be a helpful and valuable biomarker for diagnosis and prognosis of HCC.


RASSF1A Promoter methylation Hepatocellular carcinoma Meta-analysis 



We would like to acknowledge the reviewers for their helpful comments on this paper.

Conflict of interest

The authors have declared that no competing interests exist.


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Yong-Shuang Li
    • 1
    Email author
  • Qiang Xie
    • 1
  • Da-Ye Yang
    • 1
  • Yuan Zheng
    • 1
  1. 1.Department of Emergency SurgeryThe Fourth Affiliated Hospital of China Medical UniversityHuanggu DistrictPeople’s Republic of China

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