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Molecular Biology Reports

, Volume 41, Issue 5, pp 2767–2777 | Cite as

Identification of differentially expressed known and novel miRNAs in broodiness of goose

  • Fang Chen
  • Jinjun Li
  • Hao Zhang
  • Jing Xu
  • Zhengrong Tao
  • Junda Shen
  • Jianliang Shen
  • Lizhi LuEmail author
  • ChunMei LiEmail author
Article

Abstract

MicroRNAs (miRNAs) are endogenous small noncoding RNAs plays a critical role in posttranscriptional regulation of gene expression. Broodiness is observed in most avian species and influences egg production. Several genes are known to play an important role in regulating the progress of reproduction. The goose is one of the most important waterfowls. However, the involvement of miRNAs in the broodiness behavior of Anser cygnoides (Swan Goose) is unknown. High-throughput sequencing and bioinformatics analysis were used to identify the miRNAs involved in egg-laying and brooding behavior of geese in our study. The results showed 38 up-regulated and 14 down-regulated known miRNAs/miRNA*s with reads >1,000 in at least one group and a fold change of >2.0, compared with those of the egg-laying group (P < 0.001). We also identified 114 and 94 novel miRNAs in the broody and egg-laying groups, respectively. Of these, 4 novel miRNAs were differentially expressed between the two groups. The study showed the expression of small RNAs in goose reproduction and identified known and novel miRNAs regulated in broodiness. The results reveal that these differentially expressed miRNAs may be involved in broodiness of A. cygnoides.

Keywords

miRNA Goose Broodiness Egg-laying Hypothalamus 

Notes

Acknowledgments

This study was supported by the International Science & Technology Cooperation Program of China (2013DFR30980), the earmarked fund for Modern Agro-industry Technology Research System of China (CARS-43-02) and Science and Technology Department of Zhejiang province (2012C12906-18).

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Fang Chen
    • 1
    • 2
  • Jinjun Li
    • 2
  • Hao Zhang
    • 1
    • 2
  • Jing Xu
    • 1
    • 2
  • Zhengrong Tao
    • 2
  • Junda Shen
    • 2
  • Jianliang Shen
    • 3
  • Lizhi Lu
    • 2
    Email author
  • ChunMei Li
    • 1
    Email author
  1. 1.College of Animal Sciences and TechnologyNanjing Agriculture UniversityNanjingChina
  2. 2.Institute of Animal Husbandry and Veterinary ScienceZhejiang Academy of Agricultural SciencesHangzhouChina
  3. 3.Huzhou Zhongwang Poultry Industry Co., Ltd.HuzhouChina

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