Molecular Biology Reports

, Volume 41, Issue 4, pp 1977–1984

MiR-200a is involved in proliferation and apoptosis in the human endometrial adenocarcinoma cell line HEC-1B by targeting the tumor suppressor PTEN

  • Rong Li
  • Jun-lin He
  • Xue-mei Chen
  • Chun-Lan Long
  • De-Hui Yang
  • Yu-Bin Ding
  • Hong-Bo Qi
  • Xue-Qing Liu
Article

Abstract

Abnormal cell proliferation is a main driver of tumor formation and development, which involves the deletion, mutation, and downregulation of tumor suppressor genes. One study recently demonstrated that miR-200a plays an oncogenic role by inhibiting phosphatase and tensin homolog deleted on chromosome ten (PTEN) expression. In the human endometrial adenocarcinoma cell line HEC-1B, suppression of miR-200a expression inhibited cell proliferation and promoted apoptosis, whereas its over-expression had no effect on proliferation and apoptosis. Furthermore, inhibition or over-expression of miR-200a increased or reduced the expression of PTEN, respectively, with no change in PTEN mRNA levels. These effects were achieved by directly targeting miR-200a to the 3′ untranslated region of the PTEN mRNA to inhibit its translation. Taken together, we propose that in HEC-1B cells, miR-200a functions as an oncogene, affecting proliferation and apoptosis by regulating the expression of the tumor suppressor PTEN at the translational level.

Keywords

HEC-1B miR-200a PTEN Proliferation Apoptosis 

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Rong Li
    • 1
    • 2
  • Jun-lin He
    • 1
  • Xue-mei Chen
    • 1
  • Chun-Lan Long
    • 3
  • De-Hui Yang
    • 1
  • Yu-Bin Ding
    • 1
    • 2
  • Hong-Bo Qi
    • 2
  • Xue-Qing Liu
    • 1
  1. 1.Laboratory of Reproductive BiologyChongqing Medical UniversityChongqingPeople’s Republic of China
  2. 2.Department of Obstetrics and GynecologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqingPeople’s Republic of China
  3. 3.Pediatric Research Institute of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and DisordersChildren’s Hospital of Chongqing Medical UniversityChongqingPeople’s Republic of China

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