Molecular Biology Reports

, Volume 41, Issue 3, pp 1799–1805 | Cite as

The mitochondrial 16 s rRNA reveals high anthropogenic influence on land snail diversity in a preliminary island survey

  • Siti-Balkhis Abu-Bakar
  • Norhanis M. Razali
  • Fred Naggs
  • Christopher Wade
  • Siti-Azizah Mohd-Nor
  • Shau-Hwai Aileen-Tan


A total of 30 specimens belonging to five species, namely; Cryptozona siamensis, Sarika resplendens and Sarika sp. from the family Ariophantidae as well as Quantula striata and Quantula sp. from the family Dyakiidae were collected from the Langkawi Island in Northern Peninsular Malaysia. All specimens were identified through comparisons of shell morphology and amplification of a 500 bp segment of the 16S rRNA mtDNA gene. To assess phylogenetic insights, the sequences were aligned using ClustalW and phylogenetic trees were constructed. The analyses showed two major lineages in both Maximum Parsimony and Neighbour Joining phylogenetic trees. Each putative taxonomic group formed a monophyletic cluster. Our study revealed low species and intraspecies genetic diversities based on the 16S rRNA gene sequences. Thus, this study has provided an insight of land snail diversity in populations of an island highly influenced by anthropogenic activities through complementary use of shell morphological and molecular data.


Land snails Stylommatophora 16S rRNA Molecular phylogeny Ariophantidae Dyakiidae 



We would like to thank all colleagues from Marine Science Lab, Universiti Sains Malaysia (USM) who are involved in specimen collection, and Natural History Museum London, School of Biology University of Nottingham, and Molecular Ecology Research Group, USM for assistance during the laboratorial work. This study was partially funded by Universiti Sains Malaysia Research University Grant (1001/PBiologi/833025) and British Council Connect Prime Minister’s Initiative 2 (PMI2) Grant and Universiti Sains Malaysia Fellowship to the first author.


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Siti-Balkhis Abu-Bakar
    • 1
  • Norhanis M. Razali
    • 1
  • Fred Naggs
    • 2
  • Christopher Wade
    • 3
  • Siti-Azizah Mohd-Nor
    • 1
  • Shau-Hwai Aileen-Tan
    • 1
  1. 1.School of Biological SciencesUniversiti Sains MalaysiaPenangMalaysia
  2. 2.Department of ZoologyThe Natural History MuseumLondonUK
  3. 3.School of BiologyUniversity of NottinghamNottinghamUK

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