Molecular Biology Reports

, Volume 41, Issue 2, pp 741–749 | Cite as

Bioceramic-collagen scaffolds loaded with human adipose-tissue derived stem cells for bone tissue engineering

  • Neda Daei-farshbaf
  • Abdolreza Ardeshirylajimi
  • Ehsan Seyedjafari
  • Abbas Piryaei
  • Fatemeh Fadaei Fathabady
  • Mehdi Hedayati
  • Mohammad Salehi
  • Masoud Soleimani
  • Hamid Nazarian
  • Sadegh-Lotfalah Moradi
  • Mohsen NorouzianEmail author


The combination of bioceramics and stem cells has attracted the interest of research community for bone tissue engineering applications. In the present study, a combination of Bio-Oss® and type 1 collagen gel as scaffold were loaded with human adipose-tissue derived mesenchymal stem cells (AT-MSCs) after isolation and characterization, and the capacity of them for bone regeneration was investigated in rat critical size defects using digital mammography, multi-slice spiral computed tomography imaging and histological analysis. 8 weeks after implantation, no mortality or sign of inflammation was observed in the site of defect. According to the results of imaging analysis, a higher level of bone regeneration was observed in the rats receiving Bio-Oss®-Gel compared to untreated group. In addition, MSC-seeded Bio-Oss-Gel induced the highest bone reconstruction among all groups. Histological staining confirmed these findings and impressive osseointegration was observed in MSC-seeded Bio-Oss-Gel compared with Bio-Oss-Gel. On the whole, it was demonstrated that combination of AT-MSCs, Bio-Oss and Gel synergistically enhanced bone regeneration and reconstruction and also could serve as an appropriate structure to bone regenerative medicine and tissue engineering application.


Mesenchymal stem cells Tissue engineering Bone Bioceramic Critical-size defect 


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Neda Daei-farshbaf
    • 1
  • Abdolreza Ardeshirylajimi
    • 2
  • Ehsan Seyedjafari
    • 3
  • Abbas Piryaei
    • 1
  • Fatemeh Fadaei Fathabady
    • 1
  • Mehdi Hedayati
    • 4
  • Mohammad Salehi
    • 5
  • Masoud Soleimani
    • 6
  • Hamid Nazarian
    • 1
  • Sadegh-Lotfalah Moradi
    • 2
  • Mohsen Norouzian
    • 1
    Email author
  1. 1.Departments of Anatomy and Cell BiologyShahid Beheshti University of Medical SciencesTehranIran
  2. 2.Departments of Stem Cell BiologyStem Cell Technology Research CenterTehranIran
  3. 3.Department of Biotechnology, College of ScienceUniversity of TehranTehranIran
  4. 4.Endocrine Research CenterShahid Beheshti University of Medical SciencesTehranIran
  5. 5.Department of Biotechnology, Faculty of MedicineShahid Beheshti University of Medical SciencesTehranIran
  6. 6.Department of Hematology, Faculty of Medical ScienceTarbiat Modares UniversityTehranIran

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