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Molecular Biology Reports

, Volume 41, Issue 2, pp 671–681 | Cite as

Proteome analysis of roots of wheat seedlings under aluminum stress

  • Myeong Won Oh
  • Swapan Kumar Roy
  • Abu Hena Mostofa Kamal
  • Kun Cho
  • Seong-Woo Cho
  • Chul-Soo Park
  • Jong-Soon Choi
  • Setsuko Komatsu
  • Sun-Hee WooEmail author
Article

Abstract

The root apex is considered the first sites of aluminum (Al) toxicity and the reduction in root biomass leads to poor uptake of water and nutrients. Aluminum is considered the most limiting factor for plant productivity in acidic soils. Aluminum is a light metal that makes up 7 % of the earth’s scab dissolving ionic forms. The inhibition of root growth is recognized as the primary effect of Al toxicity. Seeds of wheat cv. Keumkang were germinated on petridish for 5 days and then transferred hydroponic apparatus which was treated without or with 100 and 150 μM AlCl3 for 5 days. The length of roots, shoots and fresh weight of wheat seedlings were decreased under aluminum stress. The concentration of K+, Mg2+ and Ca2+ were decreased, whereas Al3+ and P2O5 concentration was increased under aluminum stress. Using confocal microscopy, the fluorescence intensity of aluminum increased with morin staining. A proteome analysis was performed to identify proteins, which are responsible to aluminum stress in wheat roots. Proteins were extracted from roots and separated by 2-DE. A total of 47 protein spots were changed under Al stress. Nineteen proteins were significantly increased such as sadenosylmethionine, oxalate oxidase, malate dehydrogenase, cysteine synthase, ascorbate peroxidase and/or, 28 protein spots were significantly decreased such as heat shock protein 70, O-methytransferase 4, enolase, and amylogenin. Our results highlight the importance and identification of stress and defense responsive proteins with morphological and physiological state under Al stress.

Keywords

Aluminum stress LTQ-FTICR-MS Proteomics Wheat root 2-DE 

Abbreviations

2-DE

Two-dimensional electrophoresis

IEF

Isoelectric focusing

IPG

Immobilized pH-gradient

LTQ-FTICR

Linear trap quandropole-fourier transform ion cyclotron resonance

Notes

Acknowledgments

We obtained financial support for this study from the AGENDA (9069532012), RDA, Korea to S. H. Woo, College of Agriculture, Life and Environments, Chungbuk National University, Korea.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Myeong Won Oh
    • 1
  • Swapan Kumar Roy
    • 1
  • Abu Hena Mostofa Kamal
    • 2
  • Kun Cho
    • 3
  • Seong-Woo Cho
    • 4
  • Chul-Soo Park
    • 5
  • Jong-Soon Choi
    • 6
  • Setsuko Komatsu
    • 2
  • Sun-Hee Woo
    • 1
    Email author
  1. 1.Department of Crop ScienceChungbuk National UniversityCheong-juKorea
  2. 2.National Institute of Crop ScienceNAROTsukubaJapan
  3. 3.Mass Spectrometry Research CenterKorea Basic Science InstituteChungbukKorea
  4. 4.Labaratory of Molecular Breeding, Arid Land Research CenterTottori UniversityTottoriJapan
  5. 5.Department of Crop, Agriculture and Life ScienceChonbuk National UniversityJeonjuKorea
  6. 6.Division of Life ScienceKorea Basic Science InstituteDaejonKorea

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