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Molecular Biology Reports

, Volume 41, Issue 4, pp 1901–1906 | Cite as

Association between melatonin receptor 1A (MTNR1A) gene single-nucleotide polymorphisms and the velvet antler yield of Sika deer

  • Fei-Fei Yang
  • Li-Jun Huo
  • Li-Guo Yang
  • Hasan Riaz
  • Li-Rong Xiong
  • Jian-Guo Chen
  • Shu-Jun Zhang
  • Jia-Jun Xiong
Article

Abstract

Melatonin, a secretion from pineal gland is ambiguously considered as the key hormone involved in regulation of the antler cycle in Sika deer. To find out more about the roles of melatonin and its receptor gene, we carried out current study to investigate the association between polymorphisms in melatonin receptor 1A (MTNR1A) gene and the antler yield from Sika deer. A total of 251 Sika deer were analyzed in this study, of which consisted of Wusan Sika deer (n = 163) and Dongfeng Sika deer (n = 88). MTNRA gene was amplified by PCR and genotyped by Polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP). Three polymorphism loci (C518T, C629G and C635T) were detected in exon2 of MTNR1A gene. The restriction site Ecol881 was used for C518T while a C629G polymorphism locus was digested with Mval restriction endonucleases. In Wusan Sika deer the allele frequencies of C and T were 0.637 and 0.363 for C518T, Also C and G alleles in C629G locus were 0.206 and 0.794. Genotypic frequencies of allele CC, CT and TT were 33.7, 59.9 and 6.4 % respectively, It showed 1.8, 37.4 and 60.7 % for frequencies of genotypes CC, CG and GG. In Dongfeng Sika deer the allele frequencies of C and T were 0.518 and 0.482 for C518T, C and G alleles were 0.375 and 0.625 for C629G. Genotypic frequencies were 10.6, 82.4 and 7.1 % for genotypes CC, CT and TT respectively, and they were 1.1, 72.7 and 26.2 % for genotypes CC, CG and GG. Among three SNPs, only C629G showed significant association (P < 0.05) with average antler yield in Wusan Sika deer, while no SNP was significant in Dongfeng Sika deer. These preliminary results implied that the identified SNPs of MTNR1A gene might influence the antler yield in Wusan Sika deer.

Keywords

Sika deer Antler yield Melatonin receptor 1A gene PCR–RFLP SSCP 

Notes

Acknowledgments

The authors wish to thank the Jingmen city Wusan Sika deer Farm in Hubei Province, Dongfeng County Sika deer Farm for their assistance on blood sample and data collection. This research was financially supported by grants from the “the Fundamental Research Funds for the Central Universities (2013PY129)”.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Fei-Fei Yang
    • 1
  • Li-Jun Huo
    • 1
  • Li-Guo Yang
    • 1
  • Hasan Riaz
    • 1
  • Li-Rong Xiong
    • 1
  • Jian-Guo Chen
    • 1
  • Shu-Jun Zhang
    • 1
  • Jia-Jun Xiong
    • 1
  1. 1.Key Laboratory of Agricultural Animal GeneticsBreeding and Reproduction, Ministry of Education, Huazhong Agricultural UniversityWuhanChina

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