Molecular Biology Reports

, Volume 41, Issue 1, pp 19–24 | Cite as

Silencing MAP3K1 expression through RNA interference enhances paclitaxel-induced cell cycle arrest in human breast cancer cells

  • Pinghua Hu
  • Qin Huang
  • Zhihua Li
  • Xiaobo Wu
  • Qianwen Ouyang
  • Jun Chen
  • Yali CaoEmail author


The objective of this study is to compare the expression level of MAP3K1 between normal mammary gland cells and breast cancer cells, and to analyze the effects of silencing MAP3K1 on breast cancer cells with paclitaxel treatment. Western blotting analysis was used to detect the expression level of MAP3K1 in MCF-7 and MCF-12F cells. The effect of gene silencing through different siRNAs was determined by realtime-PCR. MTT assay was used to test the cell proliferation. Cell cycle was detected by flow cytometry. MAP3K1 protein expression level in breast cancer cells was higher than that in normal mammary gland cells. MAP3K1 siRNA transfection significantly reduced the expression level of MAP3K1, and enhanced paclitaxel-induced cell proliferation inhibition and cell cycle arrest in breast cancer cells. Targeting MAP3K1 expression through small RNA interference can promote the therapeutic effects of paclitaxel in breast cancer.


MAP3K1 Breast cancer siRNA Cell cycle paclitaxel 


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Pinghua Hu
    • 1
  • Qin Huang
    • 1
  • Zhihua Li
    • 1
  • Xiaobo Wu
    • 1
  • Qianwen Ouyang
    • 1
  • Jun Chen
    • 1
  • Yali Cao
    • 1
    Email author
  1. 1.Jiangxi Breast Center Third Hospital of NanchangNanchangChina

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