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Molecular Biology Reports

, Volume 40, Issue 12, pp 6747–6755 | Cite as

Selection of suitable reference genes for mRNA quantification studies using common marmoset tissues

  • Yoshinori Shimamoto
  • Hiroshi Kitamura
  • Kimie Niimi
  • Yasunaga Yoshikawa
  • Fumio Hoshi
  • Mayumi Ishizuka
  • Eiki Takahashi
Article

Abstract

The common marmoset (Callithrix jacchus) is increasingly being used as a non-human primate animal model in biomedical research. To perform accurate quantitative analysis of gene expression by quantitative reverse transcription polymerase chain reaction, reliable reference genes should be selected. In this study, we evaluated the expressions of 11 widely used reference genes: ACTB, ATP5F1, B2M, GAPDH, HPRT1, PGK1, PPIA, RN18S1, RPLP0, TBP and UBC in 12 tissues and five brain areas of healthy common marmosets. NormFinder and geNorm indicated that the most suitable reference genes for cross-sectional studies of the 17 tissues were RN18S1 and RPLP0. Conversely, ACTB and PPIA were the most suitable for analyzing brain samples; however, the expression of PGK1 fluctuated among brain areas. These results indicate that suitable reference genes differ between the tissues examined. This study provides fundamental information for gene expression studies of the common marmoset and highlights the importance of validating reference genes before quantification of target mRNAs.

Keywords

Common marmoset Reference gene Housekeeping gene Quantitative RT-PCR 

Notes

Acknowledgments

This work was supported partly by a grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan to Y. S. (no. 23658255).

Supplementary material

11033_2013_2791_MOESM1_ESM.doc (7.5 mb)
Supplementary material 1 (DOC 7655 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Yoshinori Shimamoto
    • 1
  • Hiroshi Kitamura
    • 4
  • Kimie Niimi
    • 5
  • Yasunaga Yoshikawa
    • 2
  • Fumio Hoshi
    • 3
  • Mayumi Ishizuka
    • 6
  • Eiki Takahashi
    • 5
  1. 1.Department of Veterinary Teaching Hospital, School of Veterinary MedicineKitasato UniversityTowadaJapan
  2. 2.Department of Veterinary Biochemistry, School of Veterinary MedicineKitasato UniversityTowadaJapan
  3. 3.Department of Small Animal Internal Medicine, School of Veterinary MedicineKitasato UniversityTowadaJapan
  4. 4.Department of Comparative and Experimental Medicine, Graduate School of Medical SciencesNagoya City UniversityNagoyaJapan
  5. 5.Research Resources CenterRIKEN Brain Science InstituteWakoJapan
  6. 6.Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary MedicineHokkaido UniversitySapporoJapan

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