Molecular Biology Reports

, Volume 40, Issue 12, pp 6701–6708

Study of molecular mechanism of Prostaglandin E1 in inhibiting coronary heart disease

Article

DOI: 10.1007/s11033-013-2785-y

Cite this article as:
Liu, H.J., Ma, J.W., Qiao, Z.Y. et al. Mol Biol Rep (2013) 40: 6701. doi:10.1007/s11033-013-2785-y

Abstract

Prostaglandin E1 has been used clinically for improving heart diseases. In this study, we examined the effect of Prostaglandin E1 on blood lipid levels, heart protein and genes expression in coronary heart disease (CHD) rats. Female rats were fed either a control diet or hypercholesterolemic diet for 14 weeks. The feeding of a hypercholesterolemic diet (HCD) increased the serum TC, TG, and LDL-c levels, decreased the serum HDL-c, E2, P, FSH, LH and PRL levels in CHD rats. In addition, The feeding of a HCD diet markedly increased the content of serum TXA2, TXB2, and decreased the content of serum PGI2, and PGI2/TXA2, 6-Keto PGF1a. Furthermore, the feeding of a hypercholesterolemic diet markedly increased expression levels of myocardium Fas and Caspase-3 protein and mRNA levels, vascular endothelial growth factor and basic fibroblast growth factor mRNA, and decreased RyR2 mRNA in CHD rats. The feeding of Prostaglandin E1 for 14 weeks significantly reversed these abnormal biochemical indexes in rats. These findings suggest that Prostaglandin E1 play a obvious heart protective effect. The mechanisms may be related to restraining the excessive activation of Fas and Caspase-3 protein and modulating some gene expressions associated with CHD.

Keywords

Prostaglandin E1 RT-PCR VEGF mRNA bFGF mRNA Coronary heart disease Caspase-3 protein expression 

Abbreviations

TG

Triglycerides

LDL-c

Low-density lipoprotein cholesterol

HDL-c

High-density lipoprotein cholesterol

TC

Total cholesterol

E2

Estradiol

FSH

Follicular stimulating hormone

LH

Luteinizing hormone

P

Progesterone

PRL

Prolactin

TXA2

Thromboxane A2

TXB2

Thromboxane B2

PGI2

Prostaglandin analogue

VEGF

Vascular endothelial growth factor

CHD

Coronary heart disease

HCD

Hypercholesterolemic diet

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Department of CardiologyFengxian Branch of Shanghai 6th People’s HospitalShanghaiChina
  2. 2.Department of CardiologyNanjing Drum Tower Hospital, Nanjing Medical UniversityNanjingChina

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