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Molecular Biology Reports

, Volume 40, Issue 11, pp 6351–6361 | Cite as

Expression of cell cycle regulatory factors hus1, gadd45a, rb1, cdkn2a and mre11a correlates with expression of clock gene per2 in human colorectal carcinoma tissue

  • Mária Štorcelová
  • Marián Vicián
  • Richard Reis
  • Michal Zeman
  • Iveta Herichová
Article

Abstract

Deregulated expression of clock gene per2 has previously been associated with progression of cancer. The aim of the present study was to identify genes related to per2 expression and involved in cell cycle control. Patients surgically treated for colorectal carcinoma with up-regulated and down-regulated per2 expression in cancer versus adjacent tissue were studied. Total RNA from cancer tissue of these patients was used to specify genes associated with altered per2 expression using the Human Cell Cycle RT2 profiler PCR array system. We identified seven genes positively correlated (hus1, gadd45α, rb1, cdkn2a, cdk5rp1, mre11a, sumo1) and two genes negatively correlated (cdc20, birc5) with per2 expression. Expression of these seven genes was subsequently measured by real time PCR in all patients of the cohort. Patients were divided into three groups according to TNM classification. We observed an increase in gene expression in cancer tissue compared to adjacent tissue in the first group of patients in all genes measured. Expression of genes positively associated with per2 gene expression was dependent on tumor staging and changes were observed preferentially in cancer tissue. For genes negatively associated with per2 expression we also detected changes in expression dependent on tumor staging. Expression of cdc20 and birc5 was increasing in the proximal tissue and decreasing in the cancer tissue. These results implicate functional involvement of per2 in the process of carcinogenesis via newly uncovered genes. The relevancy of gene expression for determination of diagnosis and prognosis should be considered in relation to tumor staging.

Keywords

Circadian Sumo1 Cdk5rap1 Cdc20 Birc5 DNA damage 

Notes

Acknowledgments

This research was supported by Grants Slovak Research and Development Agency 0150-10 and 0291-12. The scientific grant agency of the Ministry of Education of the Slovak Republic and of the Slovak Academy of Sciences 1/1262/12.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Mária Štorcelová
    • 1
  • Marián Vicián
    • 2
  • Richard Reis
    • 2
  • Michal Zeman
    • 1
  • Iveta Herichová
    • 1
  1. 1.Department of Animal Physiology and Ethology, Faculty of Natural SciencesComenius University BratislavaBratislavaSlovak Republic
  2. 2.First Surgery DepartmentUniversity Hospital, Comenius University BratislavaBratislavaSlovak Republic

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