Molecular Biology Reports

, Volume 41, Issue 1, pp 3–8 | Cite as

The effects of platelet-rich clot releasate on the expression of MMP-1 and type I collagen in human adult dermal fibroblasts: PRP is a stronger MMP-1 stimulator

  • Min Kyung Shin
  • Jin Woo Lee
  • Young Il Kim
  • Young-Ock Kim
  • Hosik Seok
  • Nack In KimEmail author


Platelet-rich plasma is widely used in acute and chronic ulcers due to its capacity to enhance the wound healing process. Fibroblasts are believed to be the most important cells in the production and remodeling of the extracellular matrix (ECM). Matrix metalloproteinase (MMP)-1 is the proteolytic enzyme of collagen I, and has a key role in collagen remodeling during wound healing. Whether or not platelet-rich clot releasate (PRCR) is able to effectively modulate the ECM, and the effect of PRCR on the expression of type I collagen and MMP-1 in human dermal fibroblasts was evaluated. Specifically, human adult dermal fibroblasts were incubated in PRCR-containing solutions for 24 and 48 h, after which the levels of collagen and MMP-1 were quantified by reverse transcription PCR at the transcriptional level, and ELISA and immunoblot analyses at the post-transcriptional level. PRCR markedly up-regulated the expression of MMP-1 and type I collagen in fibroblasts incubated in 20 % PRCR solutions for 48 h. These findings suggest that increased MMP-1 expression after PRCR treatment enable remodeling the ECM.


Collagen Fibroblast MMP-1 Platelet-rich clot releasate Platelet-rich plasma 


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Min Kyung Shin
    • 1
  • Jin Woo Lee
    • 2
  • Young Il Kim
    • 2
  • Young-Ock Kim
    • 3
  • Hosik Seok
    • 4
  • Nack In Kim
    • 1
    Email author
  1. 1.Department of Dermatology, School of MedicineKyung Hee UniversitySeoulKorea
  2. 2.Department of Dermatology, East-West Medical Research InstitutionLoma Linda University School of MedicineLoma LindaUSA
  3. 3.Department of Herbal Crop Research, National Institute of Horticultural and Herbal ScienceRural Development AdministrationEumseongKorea
  4. 4.Kohwang Medical Research Institute, School of MedicineKyung Hee UniversitySeoulKorea

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