Molecular Biology Reports

, Volume 40, Issue 6, pp 3987–3994

Expression and tissue localization of renalase, a novel soluble FAD-dependent protein, in reproductive/steroidogenic systems

  • Mingxue Zhou
  • Tong Liang
  • Yifeng Wang
  • Da Jin
  • Jian Wang
  • Liyun Jia
  • Shuping Zhang
Article

Abstract

Renalase was initially identified in human kidney as a soluble monoamine oxidase. Here we show that renalase is predominantly expressed in reproductive/steroidogenic systems, with particularly substantial expression in oocytes, granulosa, interstitial and luteal cells of ovary, spermatogenic cells of testis, and cortex of adrenal gland, suggesting its function(s) in maturation of germ cells and steroid hormone regulation. Renalase expression increases in testes and ovaries as mice develop and its expression is further enhanced in the ovaries of pregnant mice, indicating an activity of renalase in reproduction. Gonadotropin-releasing hormone (GnRH) antagonist, cetrorelix, repressed renalase expression in mice ovaries and testes, suggesting that steroids regulate renalase expression. Leptin is an effector and modulator of steroid hormones and reproduction. Surprisingly, knockout of leptin causes a dramatic increase of renalase expression in mice testes. Taken together, our results suggest that reproductive/steroidogenic systems are also the sources for renalase secretion and renalase may play a critical role in reproduction and hormone regulation. This provides a novel insight into understanding the function of renalase.

Keywords

Renalase Expression profiling Tissue localization Reproductive/steroidogenic systems 

List of Abbreviation

NA

Noradrenaline

ADR

Adrenaline

DA

Dopamine

FAD

Flavin adenine dinucleotide

ESRD

End-stage renal disease

GnRH

Gonadotropin-releasing hormone

ob/ob

Leptin knockout

IHC

Immunohistochemistry

LH

Luteinizing hormone

FSH

Follicle-stimulating hormone

LEPR

Leptin receptor

Supplementary material

11033_2012_2476_MOESM1_ESM.doc (3.8 mb)
Supplementary material 1 (DOC 3931 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Mingxue Zhou
    • 1
    • 2
  • Tong Liang
    • 1
  • Yifeng Wang
    • 1
  • Da Jin
    • 1
  • Jian Wang
    • 1
  • Liyun Jia
    • 1
  • Shuping Zhang
    • 1
  1. 1.State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Life SciencesTsinghua UniversityBeijingChina
  2. 2.Beijing Hospital of TCM Affiliated with Capital Medical UniversityBeijing Institute of TCMBeijingChina

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