Distribution and phenotypic expression of mineralocorticoid receptor and CYP11B2 T-344C polymorphisms in a Taiwanese hypertensive population
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Numerous genetic loci are involved in the pathogenesis of hypertension, including genes related to aldosterone synthesis and mineralocorticoid receptor. The aim of this study was to evaluate the genotypic distribution of mineralocorticoid receptor and cytochrome P450 11B2 (CYP11B2) T-344C polymorphisms and their relationship with hypertension and cardiac remodeling in a Taiwanese population. Genomic DNA extracted from peripheral blood samples was subjected to polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) analysis for the mineralocorticoid receptor loci, G3514C and A4582C, and CYP11B2 T-344C. The genetic distribution and the association with echocardiographic measurements were analyzed. A total of 192 normotensive and 514 hypertensive Taiwanese patients were recruited. Statistical analysis revealed no significant differences in the genetic distribution of mineralocorticoid receptor and CYP11B2 polymorphisms between normotensive and hypertensive patients, nor were there differences in the echocardiographic measurements. Female patients with the T/T genotype of CYP11B2 were more likely to have hypertension (p = 0.045), compared with the T/C or C/C genotypes. In addition, female hypertensive patients carrying C-allele had significantly greater left ventricular mass (p = 0.0215) and left atrial dimension (p = 0.0081). Such differences were not observed in the male patients. Our data suggest that CYP11B2 T-344C polymorphism affects left ventricular structures only in the female hypertensive population. This gender-difference needs to be further elucidated.
KeywordsMineralocorticoid receptor CYP11B2 Hypertension Cardiac remodeling Left ventricular mass
This study was supported by research grants from Chung Shan Medical University Hospital, Taiwan (CSH-2009-C-002) and National Science Council, Taiwan (NSC 99-2314-B-040-020-MY2).
- 9.Russo P, Siani A, Venezia A, Iacone R, Russo O, Barba G et al (2002) Interaction between the C(-344)T polymorphism of CYP11B2 and age in the regulation of blood pressure and plasma aldosterone levels: cross-sectional and longitudinal findings of the olivetti prospective heart study. J Hypertens 20:1785–1792PubMedCrossRefGoogle Scholar
- 12.Park SH, Shub C, Nobrega TP, Bailey KR, Seward JB (1996) Two-dimensional echocardiographic calculation of left ventricular mass as recommended by the American Society of echocardiography: correlation with autopsy and M-mode echocardiography. J Am Soc Echocardiogr 9:119–128PubMedCrossRefGoogle Scholar