Molecular Biology Reports

, Volume 40, Issue 5, pp 3675–3680

Polymorphism in vitamin D receptor and osteoprotegerin genes in Egyptian rheumatoid arthritis patients with and without osteoporosis

  • Yousry Mostafa Hussien
  • Amal Shehata
  • Rehab A. Karam
  • Saad S. Alzahrani
  • Hanem Magdy
  • Abeer M. El-Shafey


1α,25-Dihydroxyvitamin D3 upregulates the expression of the receptor activator of nuclear factor kB ligand (RANKL), and downregulates osteoprotegerin (OPG) expression. We tested the effects of polymorphisms in the vitamin D receptor gene (VDR), and OPG gene in rheumatoid arthritis (RA) patients and healthy controls and their relationship to bone mineral density (BMD) and development of osteoporosis. Three hundred and fifty women were evaluated, 200 women having RA and 150 healthy control. The subjects were genotyped for polymorphism at BsmI in VDR and A163G in OPG genes by polymerase chain reaction followed by restriction fragment length polymorphism analysis. BMD was also measured. In A163G, the G allele increased the risk for RA and for the development of osteoporosis. We found a significant association between lower hip (BMD-h) and genotype variants of VDR (BsmI) and OPG A163G in RA patients with osteoporosis. Our results suggested that OPG A163G polymorphism was associated with RA susceptibility and with the development of osteoporosis in these patients. Also, VDR and OPG genes are important candidates for osteoporosis in RA patients.


Osteoprotegerin Vitamin D receptor Bone mineral density 


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Yousry Mostafa Hussien
    • 1
    • 2
  • Amal Shehata
    • 1
  • Rehab A. Karam
    • 1
  • Saad S. Alzahrani
    • 3
  • Hanem Magdy
    • 1
  • Abeer M. El-Shafey
    • 4
  1. 1.Department of Medical Biochemistry, Faculty of MedicineZagazig UniversityZagazigEgypt
  2. 2.Medical Laboratories Department Faculty of Applied Medical SciencesTaif UniversityTaifKingdom of Saudi Arabia
  3. 3.Family Medicine Department, Faculty of Applied Medical SciencesTaif UniversityTaifKingdom of Saudi Arabia
  4. 4.Rheumatology and Rehabilitation Department, Faculty of MedicineZagazig UniversityZagazigEgypt

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