Molecular Biology Reports

, Volume 40, Issue 4, pp 2887–2896 | Cite as

Comparative characterization of sweetpotato antioxidant genes from expressed sequence tags of dehydration-treated fibrous roots under different abiotic stress conditions

  • Yun-Hee Kim
  • Jae Cheol Jeong
  • Haeng-Soon Lee
  • Sang-Soo Kwak
Article
First Online:
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Accepted:

Abstract

Drought stress is one of the most adverse conditions for plant growth and productivity. The plant antioxidant system is an important defense mechanism and includes antioxidant enzymes and low-molecular weight antioxidants. Understanding the biochemical and molecular responses to drought is essential for improving plant resistance to water-limited conditions. Previously, we isolated and characterized expressed sequence tags (ESTs) from a full-length enriched cDNA library prepared from fibrous roots of sweetpotato subjected to dehydration stress (Kim et al. in BMB Rep 42:271–276, [5]). In this study, we isolated and characterized 11 sweetpotato antioxidant genes from sweetpotato EST library under various abiotic stress conditions, which included six intracellular CuZn superoxide dismutases (CuZnSOD), ascorbate peroxidase, catalase, glutathione peroxidase (GPX), glutathione-S-transferase, thioredoxin (TRX), and five extracellular peroxidase genes. The expression of almost all the antioxidant genes induced under dehydration treatments occurred in leaves, with the exception of extracellular swPB6, whereas some antioxidant genes showed increased expression levels in the fibrous roots, such as intracellular GPX, TRX, extracellular swPA4, and swPB7 genes. During various abiotic stress treatments in leaves, such as exposure to NaCl, cold, and abscisic acid, several intracellular antioxidant genes were strongly expressed compared with the expression of extracellular antioxidant genes. These results indicated that some intracellular antioxidant genes, especially swAPX1 and CuZnSOD, might be specifically involved in important defense mechanisms against oxidative stress induced by various abiotic stresses including dehydration in sweetpotato plants.

Keywords

Antioxidant genes Drought stress Environmental stress Expressed sequence tags Sweetpotato 

Abbreviations

ABA

Abscisic acid

APX

Ascorbate peroxidase

CAT

Catalase

DW

Distilled water

EST

Expressed sequence tags

GPX

Glutathione peroxidase

GST

Glutathione-S-transferase

POD

Peroxidase

ROS

Reactive oxygen species

SOD

Superoxide dismutase

TRX

Thioredoxin

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Notes

Acknowledgments

This work was supported by grants from the National Center for GM Crops (PJ008097), the Systems & Synthetic Agrobiotech Center (PJ008119), Biogreen21 Project for Next Generation, Rural Development Administration, Korea; Korea–China Joint Research Program, National Research Foundation of Korea (NRF), and the KRIBB initiative program.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Yun-Hee Kim
    • 1
  • Jae Cheol Jeong
    • 1
  • Haeng-Soon Lee
    • 1
  • Sang-Soo Kwak
    • 1
  1. 1.Environmental Biotechnology Research CenterKorea Research Institute of Bioscience and Biotechnology (KRIBB)DaejeonRepublic of Korea

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