Molecular Biology Reports

, Volume 40, Issue 2, pp 1911–1915 | Cite as

Analysis of the polymorphisms in the caprine Gli3 gene and their associations with production traits in goats

  • Q. J. Jin
  • D. X. Chen
  • L. Yang
  • X. T. Fang
  • C. L. Zhang
  • C. Z. Lei
  • H. Chen
Article
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Abstract

Gli3 is a zinc finger transcription factor which plays a critical role in regulating animal development, metabolism and energy partitioning and thus has the potential to influence economical important traits in farm animals. In this study, we screened the complete exons of the caprine Gli3 gene using PCR-SSCP methods in 430 individuals from three goat breeds to identify sequence variants that might be associated with growth traits. Six novel mutations (GU363952:g.739C>G, 749A>T, 1636C>A, 1982delT, 1983T>C, 2856T>C) were identified. Significant associations were observed between the mutations GU363952:g.739C>G and g.749A>T with body height, chest circumference and canon circumference. Individuals with genotype G4-CC/AA and G4-CG/AT were significantly higher than individuals with genotype G4-GG/TT in body height, chest circumference and canon circumference. The results of this study suggested that the Gli3-gene-specific SNP could be a useful marker for growth traits in future marker-assisted selection programs in goat.

Keywords

Goat Gli3 gene Polymorphism SSCP SNP 
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Notes

Acknowledgments

This work was supported by A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Agricultural Science and Technology Innovation Projects of Shaanxi Province (No. 2012NKC01-13). Natural Science Foundation of Jiangsu Province (No. BK2008120).

Supplementary material

11033_2012_2247_MOESM1_ESM.doc (62 kb)
Supplementary material 1 (DOC 62 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Q. J. Jin
    • 1
    • 2
  • D. X. Chen
    • 1
  • L. Yang
    • 1
    • 2
  • X. T. Fang
    • 1
  • C. L. Zhang
    • 1
  • C. Z. Lei
    • 3
  • H. Chen
    • 1
  1. 1.Institute of Cellular and Molecular BiologyJiangsu Normal UniversityXuzhouPeople’s Republic of China
  2. 2.College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge TechniqueNanjing Agricultural University, and Beckman Coulter Ltd. Co., Nanjing Agricultural UniversityNanjingPeople’s Republic of China
  3. 3.College of Animal Science and TechnologyNorthwest A&F University, Shaanxi Key Laboratory of Molecular Biology for AgricultureYanglingPeople’s Republic of China

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