Molecular Biology Reports

, Volume 39, Issue 12, pp 11217–11223

Interactions of interleukin-12A and interleukin-12B polymorphisms on the risk of intracranial aneurysm

  • Li-Juan Li
  • Xin-Min Pan
  • Xiutian Sima
  • Zhao-Hui Li
  • Lu-Shun Zhang
  • Hong Sun
  • Yi Zhu
  • Wei-Bo Liang
  • Lin-Bo Gao
  • Lin Zhang
Article

Abstract

Several lines of evidence indicate that inflammatory processes play pivotal role in the development of intracranial aneurysm (IA). Recently, polymorphisms in the interleukin-12 (IL-12) gene were shown to be associated with immune-mediated inflammatory disease. The aim of this study was to investigate the interactions of IL-12A and IL-12B polymorphisms on the risk of IA in a Chinese population. A total of 422 individuals (including 164 patients with IA and 258 controls) were involved in the study. The polymorphisms (i.e., rs2243115 and rs568408 in IL-12A and rs3212227 in IL-12B) were genotyped by polymerase chain reaction–restriction fragment length polymorphism assay and DNA sequencing. We found an association of the AC/CC genotypes and C allele of IL-12B rs3212227 with an increased risk of IA, compared with the AA genotype and A allele (AC/CC vs. AA: OR = 2.09, 95 % CI: 1.29–3.38; C vs. A: OR = 1.45, 95 % CI: 1.10–1.91). Moreover, a significant gene interaction of IL-12A and IL-12B was evident on the risk of IA, and subjects carrying variant genotypes of IL-12B rs3212227 had an increased risk of IA. In the stratified analysis by gender, the IL-12B rs3212227 AC/CC genotypes had an increased risk of IA compared with the AA genotype in male patients (AC/CC vs. AA: OR = 4.63, 95 % CI: 1.92–11.16). These findings suggest that the IL-12A and IL-12B independently and jointly be involved in the susceptibility to IA.

Keywords

Intracranial aneurysm Interleukin-12A Interleukin-12B Inflammation Genetic polymorphism 

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Li-Juan Li
    • 1
  • Xin-Min Pan
    • 2
  • Xiutian Sima
    • 3
  • Zhao-Hui Li
    • 4
  • Lu-Shun Zhang
    • 1
  • Hong Sun
    • 5
  • Yi Zhu
    • 1
  • Wei-Bo Liang
    • 1
  • Lin-Bo Gao
    • 5
    • 6
  • Lin Zhang
    • 1
  1. 1.Department of Forensic BiologyWest China School of Preclinical and Forensic Medicine, Sichuan UniversityChengduPeople’s Republic of China
  2. 2.Department of Forensic PathologyHenan University of Science and TechnologyLuoyangPeople’s Republic of China
  3. 3.Department of NeurosurgeryWest China Hospital of Sichuan UniversityChengduPeople’s Republic of China
  4. 4.Department of Gastrointestinal and Pancreatic SurgeryLuoyang Central HospitalLuoyangPeople’s Republic of China
  5. 5.Laboratory of Molecular and Translational MedicineWest China Institute of Women and Children’s Health, West China Second University Hospital, Sichuan UniversityChengduPeople’s Republic of China
  6. 6.Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of EducationChengduPeople’s Republic of China

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