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Molecular Biology Reports

, Volume 39, Issue 10, pp 9755–9764 | Cite as

Sterol glycosyltransferases-identification of members of gene family and their role in stress in Withania somnifera

  • Pankaj Chaturvedi
  • Manoj Mishra
  • Nehal Akhtar
  • Parul Gupta
  • Pratibha Mishra
  • Rakesh Tuli
Article

Abstract

Sterol glycosyltransferases (SGTs) catalyze the transfer of sugar molecules to diverse sterol molecules, leading to a change in their participation in cellular metabolism. Withania somnifera is a medicinal plant rich in sterols, sterol glycosides and steroidal lactones. Sterols and their modified counterparts are medicinally important and play a role in adaptation of the plant to stress conditions. We have identified 3 members of SGT gene family through RACE (Rapid Amplification of cDNA Ends) in addition to sgtl1 reported earlier. The amino acid sequence deduced from the ORF’s showed homology (45–67 %) to the reported plant SGTs. The expression of the genes was differentially modulated in different organs in W. somnifera and in response to external stimuli. Salicylic acid and methyl jasmonate treatments showed up to 10 fold increase in the expression of sgt genes suggesting their role in defense. The level of expression increased in heat and cold stress indicating the role of sterol modifications in abiotic stress. One of the members, was expressed in E. coli and the enzyme assay showed that the crude enzyme glycosylated stigmasterol. W. somnifera expresses a family of sgt genes and there is a functional recruitment of these genes under stress conditions. The genes which are involved in sterol modification are important in view of medicinal value and understanding stress.

Keywords

Cold stress Glycosyltransferases Heat stress Medicinal plants Saponins Sterols 

Notes

Acknowledgments

We are grateful to the Council of Scientific and Industrial Research, Government of India for financial support to the project under New Millennium Indian Technology Leadership Initiative.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Pankaj Chaturvedi
    • 1
  • Manoj Mishra
    • 2
  • Nehal Akhtar
    • 2
  • Parul Gupta
    • 2
  • Pratibha Mishra
    • 2
  • Rakesh Tuli
    • 3
  1. 1.Sanjay Gandhi Post Graduate Institute of Medical SciencesLucknowIndia
  2. 2.Tissue Culture and Transformation LaboratoryNational Botanical Research Institute (Council of Scientific & Industrial Research)LucknowIndia
  3. 3.National Agri-food Biotechnology InstituteMohaliIndia

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