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Molecular Biology Reports

, Volume 39, Issue 9, pp 8907–8918 | Cite as

Isolation and characterization of two ABRE-binding proteins: EABF and EABF1 from the oil palm

  • Vahid Omidvar
  • Siti Nor Akmar AbdullahEmail author
  • Chai Ling Ho
  • Maziah Mahmood
  • Ahmed Bakhit Al-Shanfari
Article

Abstract

Abscisic acid (ABA) is an important phytohormone involved in the abiotic stress resistance in plants. The ABA-responsive element (ABRE) binding factors play significant roles in the plant development and response to abiotic stresses, but none so far have been isolated and characterized from the oil palm. Two ABA-responsive cDNA clones, named EABF and EABF1, were isolated from the oil palm fruits using yeast one-hybrid system. The EABF had a conserved AP2/EREBP DNA-binding domain (DNA-BD) and a potential nuclear localization sequence (NLS). No previously known DNA-BD was identified from the EABF1 sequence. The EABF and EABF1 proteins were classified as DREB/CBF and bZIP family members based on the multiple sequence alignment and phylogenetic analysis. Both proteins showed ABRE-binding and transcriptional activation properties in yeast. Furthermore, both proteins were able to trans-activate the down-stream expression of the LacZ reporter gene in yeast. An electrophoretic mobility shift assay revealed that in addition to the ABRE sequence, both proteins could bind to the DRE sequence as well. Transcriptional analysis revealed that the expression of EABF was induced in response to the ABA in the oil palm fruits and leaves, but not in roots, while the EABF1 was constitutively induced in all tissues. The expressions of both genes were strongly induced in fruits in response to the ABA, ethylene, methyl jasmonate, drought, cold and high-salinity treatments, indicating that the EABF and EABF1 might act as connectors among different stress signal transduction pathways. Our results indicate that the EABF and EABF1 are novel stress-responsive transcription factors, which are involved in the abiotic stress response and ABA signaling in the oil palm and could be used for production of stress-tolerant transgenic crops.

Keywords

Abscisic acid responsive element Abiotic stress Transcription factor Yeast one-hybrid 

Abbreviations

ABA

Abscisic acid

ABRE

ABA-responsive element

ABF

ABRE-binding factor

DRE

Dehydration-responsive element

CRT

C-repeat

DREB

DRE-binding protein

CBF

C-repeat binding factor

EMSA

Electrophoretic mobility shift assay

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Vahid Omidvar
    • 1
    • 2
  • Siti Nor Akmar Abdullah
    • 1
    • 2
    Email author
  • Chai Ling Ho
    • 3
  • Maziah Mahmood
    • 3
  • Ahmed Bakhit Al-Shanfari
    • 1
    • 2
  1. 1.Department of Agriculture Technology, Faculty of AgricultureUniversity Putra Malaysia, UPMSelangorMalaysia
  2. 2.Laboratory of Plantation CropsInstitute of Tropical Agriculture, UPMSelangorMalaysia
  3. 3.Department of Cell and Molecular BiologyFaculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPMSelangorMalaysia

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