Molecular Biology Reports

, Volume 39, Issue 9, pp 8793–8802 | Cite as

Biomimetic synthesis and characterization of cobalt nanoparticles using apoferritin, and investigation of direct electron transfer of Co(NPs)–ferritin at modified glassy carbon electrode to design a novel nanobiosensor

  • Soheila Kashanian
  • Fereshteh Abasi Tarighat
  • Ronak Rafipour
  • Maryam Abbasi-Tarighat
Article

Abstract

Oxyhydroxy cobalt CoO(OH) nanoparticles (Co-NPs) were prepared in horse spleen apoferritin (HsAFr) cavity. Transmission electron microscopy revealed the particle size was 5.5–6 nm. Mineralization effect on HsAFr was investigated by fluorescence and far-UV circular dichroism (far-UV CD) spectroscopies. The far-UV CD experiments indicated an increase in the α-helical content after mineralization. Intrinsic fluorescence data showed that mineralization acts as a quencher of HsAFr. For the first time, direct electron transfer between Co(NPs)–HsAFr and a glassy carbon electrode in the thin film of dihexadecylphosphate (DHP) was investigated by cyclic voltammetry (CV) to design a biosensor. The anionic surfactant DHP was used to achieve direct electron-transfer between Co(NPs)–HsAFr molecules and the GC electrode surface. CV result showed clearly a pair of well-defined and quasi-reversible redox peaks arise from Co(NPs)–HsAFr embedded in DHP film. This novel biosensor can be used in medical and industrial fields to detect different analytes.

Keywords

Nanoparticles Biosensor Direct electron transfer 

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Soheila Kashanian
    • 1
  • Fereshteh Abasi Tarighat
    • 2
  • Ronak Rafipour
    • 3
  • Maryam Abbasi-Tarighat
    • 4
  1. 1.Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC)Razi UniversityKermanshahIslamic Republic of Iran
  2. 2.Department of Biology, Faculty of Science Razi UniversityKermanshahIslamic Republic of Iran
  3. 3.Faculty of ChemistryRazi UniversityKermanshahIslamic Republic of Iran
  4. 4.Department of ChemistryFaculty of Sciences, Persian Gulf UniversityBushehrIslamic Republic of Iran

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